Interactions between hydrogen sulphide and rhizobia modulate the physiological and metabolism process during water deficiency-induced oxidative defense in soybean

Xue Yuan Lin, Ni Na Zhang, Bai Hui Yao, Xin Zhang, Wu Yu Liu, Wei Qin Zhang, Jian Hua Zhang, Ge Hong Wei, Juan Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Hydrogen sulphide (H2S), a new gas signal molecule, participates in the regulation of various abiotic stresses in plants. However, how the tandem working of H2S and rhizobia affects the adaptation of soybean to water deficiency is still unclear. In this study, we investigated the adaptation mechanism of H2S and rhizobia in soybean to water deficiency. Our results revealed that H2S and rhizobia jointly enhanced the leaf chlorophyll content and relative water content in plants, and caused an increase in the biomass of soybean seedlings under water deficiency. Besides, in the absence of water, H2S enhanced the biomass by affecting the number of nodules and nitrogenase activity during vegetative growth. The expression of nodulation marker genes including early nodulin 40 (GmENOD40), ERF required for nodulation (GmERN) and nodulation inception genes (GmNIN1a, GmNIN2a and GmNIN2b) were upregulated by H2S and rhizobia in the nodules. Moreover, the combined effect of H2S and rhizobia was proved to affect the enzyme activities and gene expression level of antioxidants, as well as osmotic protective substance content and related gene expression levels under water deficiency in soybean seedlings. In addition, the metabolomic results suggested that the combined effect of H2S and rhizobia remarkably promoted the contents of lipids and lipid-like molecules. Our results indicated that H2S and rhizobia synergistically reduced the oxidative damage caused by water deficiency through increasing the accumulation of metabolites and strengthening the plant antioxidant capacity.

Original languageEnglish
Pages (from-to)3249-3274
Number of pages26
JournalPlant Cell and Environment
Volume45
Issue number11
DOIs
Publication statusPublished - Nov 2022

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • antioxidant defense
  • hydrogen sulphide (H2S)
  • metabolomics
  • osmotic adjustment
  • rhizobia
  • soybean (Glycine max)

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