Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis

Juan Fang Wu, Ling Xin Chen, Guo An Luo*, Yong Suo Liu, Yi Ming Wang, Zhi Hong JIANG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Two non-self-complementary 17-mer double-stranded DNA (dsDNA) with four different central base pairs were designed to systematically investigate the binding affinity and sequence specificity of berberine with dsDNA by capillary zone electrophoresis (CZE). The data analysis with the Kenndler model proved only low affinity between dsDNA and berberine and suggested some weak binding preference of berberine for AATT-containing to GGCC-containing dsDNA. The binding constant, Ka, between berberine and dsDNAAB was about (1.0 ± 0.7) × 103 M-1. In addition, the separation of single-stranded DNA (ssDNA) from dsDNA under simple electrophoretic conditions enabled CZE to be a potentially alternative tool to check the extent of DNA annealing, which is usually done by the time-consuming and labor-intensive slab electrophoresis.

Original languageEnglish
Pages (from-to)158-164
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume833
Issue number2
DOIs
Publication statusPublished - 3 Apr 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

User-Defined Keywords

  • Berberine
  • Capillary zone electrophoresis
  • DNA
  • DNA annealing
  • Interaction

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