Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis

Juan Fang Wu; Ling Xin Chen; Guo An Luo; Yong Suo Liu; Yi Ming Wang; Zhi Hong JIANG

doi:10.1016/j.jchromb.2006.01.028

Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis

Juan Fang Wu, Ling Xin Chen, Guo An Luo^*, Yong Suo Liu, Yi Ming Wang, Zhi Hong JIANG

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Journal article › peer-review

12 Citations (Scopus)

Abstract

Two non-self-complementary 17-mer double-stranded DNA (dsDNA) with four different central base pairs were designed to systematically investigate the binding affinity and sequence specificity of berberine with dsDNA by capillary zone electrophoresis (CZE). The data analysis with the Kenndler model proved only low affinity between dsDNA and berberine and suggested some weak binding preference of berberine for AATT-containing to GGCC-containing dsDNA. The binding constant, K_a, between berberine and dsDNA_AB was about (1.0 ± 0.7) × 10³ M^-1. In addition, the separation of single-stranded DNA (ssDNA) from dsDNA under simple electrophoretic conditions enabled CZE to be a potentially alternative tool to check the extent of DNA annealing, which is usually done by the time-consuming and labor-intensive slab electrophoresis.

Original language	English
Pages (from-to)	158-164
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	833
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2006.01.028
Publication status	Published - 3 Apr 2006

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

User-Defined Keywords

Berberine
Capillary zone electrophoresis
DNA
DNA annealing
Interaction

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10.1016/j.jchromb.2006.01.028

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@article{4308a43b742646959f1fc615436bb071,

title = "Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis",

abstract = "Two non-self-complementary 17-mer double-stranded DNA (dsDNA) with four different central base pairs were designed to systematically investigate the binding affinity and sequence specificity of berberine with dsDNA by capillary zone electrophoresis (CZE). The data analysis with the Kenndler model proved only low affinity between dsDNA and berberine and suggested some weak binding preference of berberine for AATT-containing to GGCC-containing dsDNA. The binding constant, Ka, between berberine and dsDNAAB was about (1.0 ± 0.7) × 103 M-1. In addition, the separation of single-stranded DNA (ssDNA) from dsDNA under simple electrophoretic conditions enabled CZE to be a potentially alternative tool to check the extent of DNA annealing, which is usually done by the time-consuming and labor-intensive slab electrophoresis.",

keywords = "Berberine, Capillary zone electrophoresis, DNA, DNA annealing, Interaction",

author = "Wu, {Juan Fang} and Chen, {Ling Xin} and Luo, {Guo An} and Liu, {Yong Suo} and Wang, {Yi Ming} and JIANG, {Zhi Hong}",

note = "Funding Information: This work were financially supported by the National Natural Science Foundation of China (Grant No. 20475031) and the National “863” Project (Grant No. 2002AA2Z2004), and the Faculty Research Grants of Hong Kong Baptist University.",

year = "2006",

month = apr,

day = "3",

doi = "10.1016/j.jchromb.2006.01.028",

language = "English",

volume = "833",

pages = "158--164",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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T1 - Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis

AU - Wu, Juan Fang

AU - Chen, Ling Xin

AU - Luo, Guo An

AU - Liu, Yong Suo

AU - Wang, Yi Ming

AU - JIANG, Zhi Hong

N1 - Funding Information: This work were financially supported by the National Natural Science Foundation of China (Grant No. 20475031) and the National “863” Project (Grant No. 2002AA2Z2004), and the Faculty Research Grants of Hong Kong Baptist University.

PY - 2006/4/3

Y1 - 2006/4/3

N2 - Two non-self-complementary 17-mer double-stranded DNA (dsDNA) with four different central base pairs were designed to systematically investigate the binding affinity and sequence specificity of berberine with dsDNA by capillary zone electrophoresis (CZE). The data analysis with the Kenndler model proved only low affinity between dsDNA and berberine and suggested some weak binding preference of berberine for AATT-containing to GGCC-containing dsDNA. The binding constant, Ka, between berberine and dsDNAAB was about (1.0 ± 0.7) × 103 M-1. In addition, the separation of single-stranded DNA (ssDNA) from dsDNA under simple electrophoretic conditions enabled CZE to be a potentially alternative tool to check the extent of DNA annealing, which is usually done by the time-consuming and labor-intensive slab electrophoresis.

AB - Two non-self-complementary 17-mer double-stranded DNA (dsDNA) with four different central base pairs were designed to systematically investigate the binding affinity and sequence specificity of berberine with dsDNA by capillary zone electrophoresis (CZE). The data analysis with the Kenndler model proved only low affinity between dsDNA and berberine and suggested some weak binding preference of berberine for AATT-containing to GGCC-containing dsDNA. The binding constant, Ka, between berberine and dsDNAAB was about (1.0 ± 0.7) × 103 M-1. In addition, the separation of single-stranded DNA (ssDNA) from dsDNA under simple electrophoretic conditions enabled CZE to be a potentially alternative tool to check the extent of DNA annealing, which is usually done by the time-consuming and labor-intensive slab electrophoresis.

KW - Berberine

KW - Capillary zone electrophoresis

KW - DNA

KW - DNA annealing

KW - Interaction

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U2 - 10.1016/j.jchromb.2006.01.028

DO - 10.1016/j.jchromb.2006.01.028

M3 - Journal article

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AN - SCOPUS:33645218155

SN - 1570-0232

VL - 833

SP - 158

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Interaction study between double-stranded DNA and berberine using capillary zone electrophoresis

Abstract

Scopus Subject Areas

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