Integrated Functional Omics Analysis of Flavonoid-Related Metabolism in AtMYB12 Transcript Factor Overexpressed Tomato

Xuanlin Meng, Xuanlin Meng, Xingchen Zhao, Xiangyu Ding, Yang Li, Guodong Cao, Zhaohui Chu, Xiuli Su, Yuanchen Liu, Xiangfeng Chen, Jinggong Guo, Zongwei CAI, Xinhua Ding

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Genetic engineering (GE) technology is widely used in plant modification. However, the results of modification may not exactly meet the expectations. Herein, we propose a new multi-omics method for GE plant evaluation based on the optimized use of the metID algorithm. Using this method, we found that flavonoid accumulation was at the expense of the great sacrifice of l-phenylalanine in GE tomatoes for the first time. Meanwhile, the ceramide series of sphingolipid is synthesized de novo from l-serine, and ceramides are the primary source of vesicles coated with flavonoids and secreted from the endoplasmic reticulum. Therefore, the accumulation of the ceramide series of sphingolipid changed the cell component of intracellular organelles. Furthermore, the improvement of the method allows us to identify more metabolites related to dysregulated pathways.

Original languageEnglish
Pages (from-to)6776-6787
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume68
Issue number24
DOIs
Publication statusPublished - 17 Jun 2020

Scopus Subject Areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

User-Defined Keywords

  • AtMYB12
  • co-enrichment
  • dysregulated pathways
  • genetic engineering
  • metID algorithm

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