Inhibition of shear-induced platelet aggregation in rat by tetramethylpyrazine and salvianolic acid B

Min LI*, Chi Zhao, Ricky N S WONG, Shinya Goto, Zhimin Wang, Fulong Liao

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Tetramethylpyrazine (TMP) and salvianolic acid B (SAB) are effective ingredients of Rhizoma Ligustici chuanxiong Hort. and Radix Salviae miltiorrhizae Bge., accordingly. The inhibitive effects of TMP, SAB and their combination on shear-induced platelet aggregation (SIPA) were investigated in the present study. SD rats were used as blood donors to collect anticoagulated blood, the concentration of platelet-rich-plasma (PRP) was adjusted to 5 × 105 μl. HAAKE rheometer RS 600 with sensor C60/0.5° was used as shear generator. Different doses of TMP and SAB and their combinations were added to the PRP. After constant shear of 15 Pa at 37°C for 360 seconds, PRP was transferred to a platelet aggregometer and SIPA was determined by turbidity. SIPA was inhibited by TMP and SAB in a dose-dependent manner. SIPA was decreased from 48.6 ± 4.6% of the control to 12.5 ± 2.1% in the presence of TMP (1.46 mM) and SAB (10 μM) (P < 0.0001). In conclusion, TMP and SAB have additive effects on inhibiting platelet aggregation induced by high shear stress.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalClinical Hemorheology and Microcirculation
Volume31
Issue number2
Publication statusPublished - 2004

Scopus Subject Areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

User-Defined Keywords

  • Chinese Medicine Partner (CMP)
  • Platelet aggregation
  • Salvianolic acid B (SAB)
  • Shear stress
  • Tetramethylpyrazine (TMP)

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