TY - JOUR
T1 - Inhibition of shear-induced platelet aggregation in rat by tetramethylpyrazine and salvianolic acid B
AU - LI, Min
AU - Zhao, Chi
AU - Wong, Ricky N S
AU - Goto, Shinya
AU - Wang, Zhimin
AU - Liao, Fulong
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004
Y1 - 2004
N2 - Tetramethylpyrazine (TMP) and salvianolic acid B (SAB) are effective ingredients of Rhizoma Ligustici chuanxiong Hort. and Radix Salviae miltiorrhizae Bge., accordingly. The inhibitive effects of TMP, SAB and their combination on shear-induced platelet aggregation (SIPA) were investigated in the present study. SD rats were used as blood donors to collect anticoagulated blood, the concentration of platelet-rich-plasma (PRP) was adjusted to 5 × 105 μl. HAAKE rheometer RS 600 with sensor C60/0.5° was used as shear generator. Different doses of TMP and SAB and their combinations were added to the PRP. After constant shear of 15 Pa at 37°C for 360 seconds, PRP was transferred to a platelet aggregometer and SIPA was determined by turbidity. SIPA was inhibited by TMP and SAB in a dose-dependent manner. SIPA was decreased from 48.6 ± 4.6% of the control to 12.5 ± 2.1% in the presence of TMP (1.46 mM) and SAB (10 μM) (P < 0.0001). In conclusion, TMP and SAB have additive effects on inhibiting platelet aggregation induced by high shear stress.
AB - Tetramethylpyrazine (TMP) and salvianolic acid B (SAB) are effective ingredients of Rhizoma Ligustici chuanxiong Hort. and Radix Salviae miltiorrhizae Bge., accordingly. The inhibitive effects of TMP, SAB and their combination on shear-induced platelet aggregation (SIPA) were investigated in the present study. SD rats were used as blood donors to collect anticoagulated blood, the concentration of platelet-rich-plasma (PRP) was adjusted to 5 × 105 μl. HAAKE rheometer RS 600 with sensor C60/0.5° was used as shear generator. Different doses of TMP and SAB and their combinations were added to the PRP. After constant shear of 15 Pa at 37°C for 360 seconds, PRP was transferred to a platelet aggregometer and SIPA was determined by turbidity. SIPA was inhibited by TMP and SAB in a dose-dependent manner. SIPA was decreased from 48.6 ± 4.6% of the control to 12.5 ± 2.1% in the presence of TMP (1.46 mM) and SAB (10 μM) (P < 0.0001). In conclusion, TMP and SAB have additive effects on inhibiting platelet aggregation induced by high shear stress.
KW - Chinese Medicine Partner (CMP)
KW - Platelet aggregation
KW - Salvianolic acid B (SAB)
KW - Shear stress
KW - Tetramethylpyrazine (TMP)
UR - https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch693
UR - http://www.scopus.com/inward/record.url?scp=4644325763&partnerID=8YFLogxK
M3 - Journal article
C2 - 15310944
AN - SCOPUS:4644325763
SN - 1386-0291
VL - 31
SP - 97
EP - 103
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
IS - 2
ER -