Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)

Chen Zhu, Wei Liu, Chaohua Luo, Yi Liu, Chan Li, Miao Fang, Yingbo Lin, Jinying Ou, Minting Chen, Daoqi Zhu, Kin Lam YUNG, Zhixian Mo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40 μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150 μg/g), high dose of rhynchophylline (100 μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.

Original languageEnglish
Pages (from-to)47-51
Number of pages5
JournalFitoterapia
Volume117
DOIs
Publication statusPublished - 1 Mar 2017

Scopus Subject Areas

  • Pharmacology
  • Drug Discovery

User-Defined Keywords

  • Methamphetamine dependence
  • Rhynchophylline
  • Tyrosine hydroxylase (TH)

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