TY - JOUR
T1 - Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)
AU - Zhu, Chen
AU - Liu, Wei
AU - Luo, Chaohua
AU - Liu, Yi
AU - Li, Chan
AU - Fang, Miao
AU - Lin, Yingbo
AU - Ou, Jinying
AU - Chen, Minting
AU - Zhu, Daoqi
AU - YUNG, Kin Lam
AU - Mo, Zhixian
N1 - Funding Information:
This work was supported by Fund Projects: the National Natural Science Foundation of China (No. 81229003, 81673628, 81403063); the Guangzhou Major Science and Technology Project (No. 201300000050); Project of Natural Science Foundation of Guangdong Province (No. 2014A030310251).
PY - 2017/3
Y1 - 2017/3
N2 - In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40 μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150 μg/g), high dose of rhynchophylline (100 μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.
AB - In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40 μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150 μg/g), high dose of rhynchophylline (100 μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.
KW - Methamphetamine dependence
KW - Rhynchophylline
KW - Tyrosine hydroxylase (TH)
UR - http://www.scopus.com/inward/record.url?scp=85009187545&partnerID=8YFLogxK
U2 - 10.1016/j.fitote.2017.01.001
DO - 10.1016/j.fitote.2017.01.001
M3 - Journal article
C2 - 28063836
AN - SCOPUS:85009187545
SN - 0367-326X
VL - 117
SP - 47
EP - 51
JO - Fitoterapia
JF - Fitoterapia
ER -