Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)

Chen Zhu; Wei Liu; Chaohua Luo; Yi Liu; Chan Li; Miao Fang; Yingbo Lin; Jinying Ou; Minting Chen; Daoqi Zhu; Kin Lam YUNG; Zhixian Mo

doi:10.1016/j.fitote.2017.01.001

Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)

Chen Zhu, Wei Liu, Chaohua Luo, Yi Liu, Chan Li, Miao Fang, Yingbo Lin, Jinying Ou, Minting Chen, Daoqi Zhu, Kin Lam YUNG^*, Zhixian Mo^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

17 Citations (Scopus)

Abstract

In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40 μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150 μg/g), high dose of rhynchophylline (100 μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.

Original language	English
Pages (from-to)	47-51
Number of pages	5
Journal	Fitoterapia
Volume	117
DOIs	https://doi.org/10.1016/j.fitote.2017.01.001
Publication status	Published - Mar 2017

User-Defined Keywords

Methamphetamine dependence
Rhynchophylline
Tyrosine hydroxylase (TH)

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10.1016/j.fitote.2017.01.001

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@article{8cb06192d51c4dbb877ec9850341bb6d,

title = "Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)",

abstract = "In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40 μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150 μg/g), high dose of rhynchophylline (100 μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.",

keywords = "Methamphetamine dependence, Rhynchophylline, Tyrosine hydroxylase (TH)",

author = "Chen Zhu and Wei Liu and Chaohua Luo and Yi Liu and Chan Li and Miao Fang and Yingbo Lin and Jinying Ou and Minting Chen and Daoqi Zhu and YUNG, {Kin Lam} and Zhixian Mo",

note = "Funding Information: This work was supported by Fund Projects: the National Natural Science Foundation of China (No. 81229003, 81673628, 81403063); the Guangzhou Major Science and Technology Project (No. 201300000050); Project of Natural Science Foundation of Guangdong Province (No. 2014A030310251).",

year = "2017",

month = mar,

doi = "10.1016/j.fitote.2017.01.001",

language = "English",

volume = "117",

pages = "47--51",

journal = "Fitoterapia",

issn = "0367-326X",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)

AU - Zhu, Chen

AU - Liu, Wei

AU - Luo, Chaohua

AU - Liu, Yi

AU - Li, Chan

AU - Fang, Miao

AU - Lin, Yingbo

AU - Ou, Jinying

AU - Chen, Minting

AU - Zhu, Daoqi

AU - YUNG, Kin Lam

AU - Mo, Zhixian

N1 - Funding Information: This work was supported by Fund Projects: the National Natural Science Foundation of China (No. 81229003, 81673628, 81403063); the Guangzhou Major Science and Technology Project (No. 201300000050); Project of Natural Science Foundation of Guangdong Province (No. 2014A030310251).

PY - 2017/3

Y1 - 2017/3

N2 - In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40 μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150 μg/g), high dose of rhynchophylline (100 μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.

AB - In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40 μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150 μg/g), high dose of rhynchophylline (100 μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.

KW - Methamphetamine dependence

KW - Rhynchophylline

KW - Tyrosine hydroxylase (TH)

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U2 - 10.1016/j.fitote.2017.01.001

DO - 10.1016/j.fitote.2017.01.001

M3 - Journal article

C2 - 28063836

AN - SCOPUS:85009187545

SN - 0367-326X

VL - 117

SP - 47

EP - 51

JO - Fitoterapia

JF - Fitoterapia

ER -

Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH)

Abstract

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