TY - JOUR
T1 - Influence of clay minerals on the Bacillus halophilus Y38 activity under anaerobic condition
AU - Zhou, Yong
AU - Chen, Huilun
AU - Yao, Jun
AU - He, Minyan
AU - Si, Yang
AU - Feng, Liang
AU - Wang, Fei
AU - Wang, Gejiao
AU - Choi, Martin M.F.
N1 - Funding Information:
We express our sincere thanks to Ms. Martina Effenberger and PD Dr. Thomas Maskow of UFZ, Department of Environmental Microbiology for their skillful technical assistance. We also thank Dr. Lixiong Han of the State Key Laboratory of Geological Processes and Mineral Resources, Chinese University of Geosciences for his supply of clay minerals and helpful discussion. This work was supported in part by grants from National Outstanding Youth Research Foundation of China (40925010), International Joint Key Project from National Natural Science Foundation of China (40920134003), National Natural Science Foundation of China (40873060, 40673065), and International Joint Key Project from Chinese Ministry of Science and Technology (2009DFA92830), and the 111 Project (B08030).
PY - 2010/12
Y1 - 2010/12
N2 - Studies of interaction between clay minerals and Bacillus halophilus Y38 have provided basic information on extremophile growth and physiological characteristics in hypersaline environments, which are of growing concern in microbiology, geobiology, environmental and pharmaceutical researches. In this study, we investigated B. halophilus Y38 strain growing in HGM hypersaline medium (sodium ion concentration >55mgmL-1, chloride ion concentration >80mgmL-1), with smectite, rectorite and kaolinite of different aggregate sizes (100μm, 100-50μm, and <50μm, respectively). Metabolic heat flow significantly increased in smaller particles, indicating that the stimulation of the B. halophilus Y38 growth by the clay minerals increased with decreasing aggregate sizes, and, thus, the close relationship between the bacterial growth and the mineral surface area. The CO2 flux (FCO2), total thermal effect (Qtotal), cell growth rate (Kcell) and protein synthesis rate (kprotein) were the key physiological parameters in metabolic and energy conservation reactions. These metabolic results showed that the stimulation by clay minerals on the B. halophilus activity was in the order smectite > rectorite > kaolinite. Our data may provide a strategy to evaluate the influence of clay minerals on the microbial growth and cell activity, and supplied a new experimental procedure to study interactions between minerals and microorganisms.
AB - Studies of interaction between clay minerals and Bacillus halophilus Y38 have provided basic information on extremophile growth and physiological characteristics in hypersaline environments, which are of growing concern in microbiology, geobiology, environmental and pharmaceutical researches. In this study, we investigated B. halophilus Y38 strain growing in HGM hypersaline medium (sodium ion concentration >55mgmL-1, chloride ion concentration >80mgmL-1), with smectite, rectorite and kaolinite of different aggregate sizes (100μm, 100-50μm, and <50μm, respectively). Metabolic heat flow significantly increased in smaller particles, indicating that the stimulation of the B. halophilus Y38 growth by the clay minerals increased with decreasing aggregate sizes, and, thus, the close relationship between the bacterial growth and the mineral surface area. The CO2 flux (FCO2), total thermal effect (Qtotal), cell growth rate (Kcell) and protein synthesis rate (kprotein) were the key physiological parameters in metabolic and energy conservation reactions. These metabolic results showed that the stimulation by clay minerals on the B. halophilus activity was in the order smectite > rectorite > kaolinite. Our data may provide a strategy to evaluate the influence of clay minerals on the microbial growth and cell activity, and supplied a new experimental procedure to study interactions between minerals and microorganisms.
KW - Clay minerals
KW - Extremophiles
KW - Microcalorimetery
KW - Salty environments
UR - http://www.scopus.com/inward/record.url?scp=78649448101&partnerID=8YFLogxK
U2 - 10.1016/j.clay.2010.10.008
DO - 10.1016/j.clay.2010.10.008
M3 - Journal article
AN - SCOPUS:78649448101
SN - 0169-1317
VL - 50
SP - 533
EP - 537
JO - Applied Clay Science
JF - Applied Clay Science
IS - 4
ER -
