Influence of bacteria and diatoms in biofilms on metamorphosis of the marine slipper limpet Crepidula onyx

Jill Man Ying Chiu, Vengatesen Thiyagarajan, Jan A. Pechenik, Oi Shing Hung, Pei Yuan Qian*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

40 Citations (Scopus)

Abstract

Larvae of the slipper limpet Crepidula onyx metamorphose in response to marine biofilms. In this study, we investigated how the percentage of larval metamorphosis in this species was affected by biofilms that differed in certain attributes. To manipulate bacterial and diatom cell densities and community composition, we developed biofilms in the laboratory (1) at different temperatures (16, 23 and 30°C) and salinities (20, 27 and 34‰), (2) with or without addition of antibiotics, and (3) in the light or in the dark. We also allowed biofilms to develop at three field sites with different prevailing environmental conditions so as to generate biofilms with different, but natural, attributes. Bacterial and diatom community composition in biofilms were determined using a DNA fingerprinting technique and microscopic examination, respectively. The effects of biofilms on metamorphosis were investigated in laboratory assays. The percentage of larval metamorphosis correlated with bacterial and diatom cell densities in only one of the three experiments conducted, but was substantially affected by differences in bacterial and diatom community composition in all three experiments. It also appears that metamorphosis of C. onyx depends on the simultaneous presence of both bacterial and diatom communities in biofilms.

Original languageEnglish
Pages (from-to)1417-1431
Number of pages15
JournalMarine Biology
Volume151
Issue number4
DOIs
Publication statusPublished - May 2007

Scopus Subject Areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

User-Defined Keywords

  • Bacterial Community
  • Community Composition
  • Onyx
  • Bacterial Community Composition
  • Diatom Community

Fingerprint

Dive into the research topics of 'Influence of bacteria and diatoms in biofilms on metamorphosis of the marine slipper limpet Crepidula onyx'. Together they form a unique fingerprint.

Cite this