Induction of tumor necrosis factor receptor type 2 gene expression by tumor necrosis factor-α in rat primary astrocytes

H. L. Lung, K. N. Leung, A. Stadlin, C. M. Ma, D. Tsang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

21 Citations (Scopus)


Using reverse transcription-polymerase chain reaction (RT-PCR) technique, the messenger RNA (mRNA) for tumor necrosis factor receptor type 2 (TNF-R2, 75/80 kDa) was detected in rat primary astrocytes, with much lower level of expression when compared to that for tumor necrosis factor receptor type 1 (TNF-R1, 55/60 kDa). Upon exposure to TNF-α (100 U/ml), the TNF-R2 mRNA level was greatly enhanced at 8 h, while TNF-R1 mRNA remained unchanged even after 24 h. The induction of TNF-R2 gene expression by TNF-α was dose-dependent and seemed to be unique to TNF-α, as interleukin-6 (IL-6) had no significant effect on TNF-R2 expression. Since TNF-R2 was reported to mediate mitogenic and gene-inducing effects in many other cell types, it is likely that the reported proliferative effect of TNF-α on astrocytes was also mediated by this TNF receptor subtype. Upon exposure to TNF-α or lipopolysaccharide (LPS), the expression of TNF-α gene was induced, and the LPS-induced TNF-α seemed to selectively enhance the TNF-R2 gene expression. Collectively, our results suggest that the TNF-α or LPS-induced expression of both TNF-R2 and TNF-α may provide a positive control mechanism to further enhance the proliferative effect of TNF-α in astrocytes.

Original languageEnglish
Pages (from-to)2081-2091
Number of pages11
JournalLife Sciences
Issue number18
Publication statusPublished - 23 Mar 2001

Scopus Subject Areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

User-Defined Keywords

  • Gene expression
  • Rat primary astrocytes
  • Receptor
  • Tumor necrosis factor-α


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