Induction of metallothionein in the Reuber H-35 rat hepatoma cell

M. S. Yang; M. K. Tang; R. N.S. Wong

doi:10.1016/0300-483X(95)03149-A

Induction of metallothionein in the Reuber H-35 rat hepatoma cell

M. S. Yang^*, M. K. Tang, R. N.S. Wong

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

7 Citations (Scopus)

Abstract

The feasibility of using the Reuber H-35 rat hepatoma cell (RH-35 cells) as model for studying metallothionein induction was examined. The RH-3S cells were treated with Cd, a toxic metal which is known to induce metallothionein. The LC₅₀ after a 3-h treatment was 70 μM. The value was significantly higher (P < 0.05) if the cells were pre-treated with a sublethal dose of CdCl₂ (5 μM) for 2 days, indicating that pre-treatment with a low dose of Cd can protect against a subsequent higher dose of the same metal. Both the mRNA and the gene product metallothionein can be identified in the cells 2 days after treatment with 5 μM Cd. In addition to Cd, Zn and Cu were also able to induce the expression of metallothionein to various degrees. The results indicate that the MT genp is present in RH-35 cells and is responsive to treatment with various metals. Thus, this cell line can be used as a model to study metallothionein induction.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Toxicology
Volume	104
Issue number	1-3
DOIs	https://doi.org/10.1016/0300-483X(95)03149-A
Publication status	Published - 15 Dec 1995

Scopus Subject Areas

Toxicology

User-Defined Keywords

Cadmium
Metallothionein induction
Reuber H-35 rat hepatoma cell

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10.1016/0300-483X(95)03149-A

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title = "Induction of metallothionein in the Reuber H-35 rat hepatoma cell",

abstract = "The feasibility of using the Reuber H-35 rat hepatoma cell (RH-35 cells) as model for studying metallothionein induction was examined. The RH-3S cells were treated with Cd, a toxic metal which is known to induce metallothionein. The LC50 after a 3-h treatment was 70 μM. The value was significantly higher (P < 0.05) if the cells were pre-treated with a sublethal dose of CdCl2 (5 μM) for 2 days, indicating that pre-treatment with a low dose of Cd can protect against a subsequent higher dose of the same metal. Both the mRNA and the gene product metallothionein can be identified in the cells 2 days after treatment with 5 μM Cd. In addition to Cd, Zn and Cu were also able to induce the expression of metallothionein to various degrees. The results indicate that the MT genp is present in RH-35 cells and is responsive to treatment with various metals. Thus, this cell line can be used as a model to study metallothionein induction.",

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author = "Yang, {M. S.} and Tang, {M. K.} and Wong, {R. N.S.}",

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T1 - Induction of metallothionein in the Reuber H-35 rat hepatoma cell

AU - Yang, M. S.

AU - Tang, M. K.

AU - Wong, R. N.S.

PY - 1995/12/15

Y1 - 1995/12/15

N2 - The feasibility of using the Reuber H-35 rat hepatoma cell (RH-35 cells) as model for studying metallothionein induction was examined. The RH-3S cells were treated with Cd, a toxic metal which is known to induce metallothionein. The LC50 after a 3-h treatment was 70 μM. The value was significantly higher (P < 0.05) if the cells were pre-treated with a sublethal dose of CdCl2 (5 μM) for 2 days, indicating that pre-treatment with a low dose of Cd can protect against a subsequent higher dose of the same metal. Both the mRNA and the gene product metallothionein can be identified in the cells 2 days after treatment with 5 μM Cd. In addition to Cd, Zn and Cu were also able to induce the expression of metallothionein to various degrees. The results indicate that the MT genp is present in RH-35 cells and is responsive to treatment with various metals. Thus, this cell line can be used as a model to study metallothionein induction.

AB - The feasibility of using the Reuber H-35 rat hepatoma cell (RH-35 cells) as model for studying metallothionein induction was examined. The RH-3S cells were treated with Cd, a toxic metal which is known to induce metallothionein. The LC50 after a 3-h treatment was 70 μM. The value was significantly higher (P < 0.05) if the cells were pre-treated with a sublethal dose of CdCl2 (5 μM) for 2 days, indicating that pre-treatment with a low dose of Cd can protect against a subsequent higher dose of the same metal. Both the mRNA and the gene product metallothionein can be identified in the cells 2 days after treatment with 5 μM Cd. In addition to Cd, Zn and Cu were also able to induce the expression of metallothionein to various degrees. The results indicate that the MT genp is present in RH-35 cells and is responsive to treatment with various metals. Thus, this cell line can be used as a model to study metallothionein induction.

KW - Cadmium

KW - Metallothionein induction

KW - Reuber H-35 rat hepatoma cell

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Induction of metallothionein in the Reuber H-35 rat hepatoma cell

Abstract

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