Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes

Rachel Carr; Robert Puckrin; Brian K. McMahon; Robert Pal; David Parker; Lars Olof Pålsson

doi:10.1088/2050-6120/2/2/024007

Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes

Rachel Carr, Robert Puckrin, Brian K. McMahon, Robert Pal, David Parker^*, Lars Olof Pålsson^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

41 Citations (Scopus)

Abstract

A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic Eu^III and Tb^IIIcomplexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, andthe observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced Tb^IIICPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 μM.

Original language	English
Article number	024007
Number of pages	7
Journal	Methods and Applications in Fluorescence
Volume	2
Issue number	2
Early online date	9 Apr 2014
DOIs	https://doi.org/10.1088/2050-6120/2/2/024007
Publication status	Published - Jun 2014

User-Defined Keywords

Biological systems
Chiral interactions
Circular polarisation
Lanthanide complexes
Optical spectroscopy

Access to Document

10.1088/2050-6120/2/2/024007

Cite this

@article{bc73818f03be4e7db834b9917463d480,

title = "Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes",

abstract = "A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic EuIII and TbIIIcomplexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, andthe observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced TbIIICPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 μM.",

keywords = "Biological systems, Chiral interactions, Circular polarisation, Lanthanide complexes, Optical spectroscopy",

author = "Rachel Carr and Robert Puckrin and McMahon, {Brian K.} and Robert Pal and David Parker and P{\aa}lsson, {Lars Olof}",

note = "We thank EPSRC and the ERC (DP, BKM, RP: FCC 266804), The Royal Society (LOP) for support and Mr Alexander G Wadham for contributions to instrument development. Dr Robert D Peacock is acknowledged for the preliminary measurements of data in figure 5. Publisher Copyright: {\textcopyright} 2014 IOP Publishing Ltd.",

year = "2014",

month = jun,

doi = "10.1088/2050-6120/2/2/024007",

language = "English",

volume = "2",

journal = "Methods and Applications in Fluorescence",

issn = "2050-6120",

publisher = "IOP Publishing Ltd.",

number = "2",

}

TY - JOUR

T1 - Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes

AU - Carr, Rachel

AU - Puckrin, Robert

AU - McMahon, Brian K.

AU - Pal, Robert

AU - Parker, David

AU - Pålsson, Lars Olof

N1 - We thank EPSRC and the ERC (DP, BKM, RP: FCC 266804), The Royal Society (LOP) for support and Mr Alexander G Wadham for contributions to instrument development. Dr Robert D Peacock is acknowledged for the preliminary measurements of data in figure 5. Publisher Copyright: © 2014 IOP Publishing Ltd.

PY - 2014/6

Y1 - 2014/6

N2 - A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic EuIII and TbIIIcomplexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, andthe observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced TbIIICPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 μM.

AB - A circularly polarized luminescence (CPL) spectrometer has been built and used to study the binding interaction of lactate and four different proteins with racemic EuIII and TbIIIcomplexes in aqueous solution. Lactate binding gives rise to strong induced CPL spectra, andthe observed emission dissymmetry factors vary linearly with enantiomeric composition. Particularly strong induced TbIIICPL also characterizes the binding interaction of alpha-1-acid glycoprotein with a dissociation constant, Kd, of 2.5 μM.

KW - Biological systems

KW - Chiral interactions

KW - Circular polarisation

KW - Lanthanide complexes

KW - Optical spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=84974530162&partnerID=8YFLogxK

U2 - 10.1088/2050-6120/2/2/024007

DO - 10.1088/2050-6120/2/2/024007

M3 - Journal article

AN - SCOPUS:84974530162

SN - 2050-6120

VL - 2

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

IS - 2

M1 - 024007

ER -

Induced circularly polarized luminescence arising from anion or protein binding to racemic emissive lanthanide complexes

Abstract

User-Defined Keywords

Access to Document

Other files and links

Fingerprint

Cite this