Indole-based Cyanine as a Nuclear RNA-Selective Two-Photon Fluorescent Probe for Live Cell Imaging

Lei Guo; Miu Shan Chan; Di Xu; Dick Yan Tam; Frédéric Bolze; Pik Kwan Lo; Man Shing Wong

doi:10.1021/cb500927r

Indole-based Cyanine as a Nuclear RNA-Selective Two-Photon Fluorescent Probe for Live Cell Imaging

Lei Guo, Miu Shan Chan, Di Xu, Dick Yan Tam, Frédéric Bolze^*, Pik Kwan Lo^*, Man Shing Wong^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

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Abstract

We have demonstrated that the subcellular targeting properties of the indole-based cyanines can be tuned by the functional substituent attached onto the indole moiety in which the first example of a highly RNA-selective and two-photon active fluorescent light-up probe for high contrast and brightness TPEF images of rRNA in the nucleolus of live cells has been developed. It is important to find that this cyanine binds much stronger toward RNA than DNA in a buffer solution as well as selectively stains and targets to rRNA in the nucleolus. Remarkably, the TPEF brightness (Φσ_max) is dramatically increased with 11-fold enhancement in the presence of rRNA, leading to the record high Φσ_max of 228 GM for RNA. This probe not only shows good biocompatibility and superior photostability but also offers general applicability to various live cell lines including HeLa, HepG2, MCF-7, and KB cells and excellent counterstaining compatibility with commercially available DNA or protein trackers.

Original language	English
Pages (from-to)	1171-1175
Number of pages	5
Journal	ACS Chemical Biology
Volume	10
Issue number	5
Early online date	19 Feb 2015
DOIs	https://doi.org/10.1021/cb500927r
Publication status	Published - 15 May 2015

Scopus Subject Areas

Biochemistry
Molecular Medicine

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title = "Indole-based Cyanine as a Nuclear RNA-Selective Two-Photon Fluorescent Probe for Live Cell Imaging",

abstract = "We have demonstrated that the subcellular targeting properties of the indole-based cyanines can be tuned by the functional substituent attached onto the indole moiety in which the first example of a highly RNA-selective and two-photon active fluorescent light-up probe for high contrast and brightness TPEF images of rRNA in the nucleolus of live cells has been developed. It is important to find that this cyanine binds much stronger toward RNA than DNA in a buffer solution as well as selectively stains and targets to rRNA in the nucleolus. Remarkably, the TPEF brightness (Φσmax) is dramatically increased with 11-fold enhancement in the presence of rRNA, leading to the record high Φσmax of 228 GM for RNA. This probe not only shows good biocompatibility and superior photostability but also offers general applicability to various live cell lines including HeLa, HepG2, MCF-7, and KB cells and excellent counterstaining compatibility with commercially available DNA or protein trackers.",

author = "Lei Guo and Chan, {Miu Shan} and Di Xu and Tam, {Dick Yan} and Fr{\'e}d{\'e}ric Bolze and Lo, {Pik Kwan} and Wong, {Man Shing}",

note = "Funding information: This work was financially supported by GRF (HKBU 203212), Hong Kong Research Grant Council, FRG (FRG2/13-14/059), Hong Kong Baptist University, the PROCORE-France/Hong Kong Joint Research Scheme sponsored by the Research Grants Council of Hong Kong and the Consulate General of France in Hong Kong, F-HK010/127 and Institute of Molecular Functional Materials which was supported by a grant from the University Grants Committee, Areas of Excellence Scheme (AoE/P-03/08). This work was also supported by CityU Strategic Research Grant 9616302 and 7004026, and National Science Foundation of China 21324077. Publisher copyright: {\textcopyright} 2015 American Chemical Society",

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AU - Bolze, Frédéric

AU - Lo, Pik Kwan

AU - Wong, Man Shing

N1 - Funding information: This work was financially supported by GRF (HKBU 203212), Hong Kong Research Grant Council, FRG (FRG2/13-14/059), Hong Kong Baptist University, the PROCORE-France/Hong Kong Joint Research Scheme sponsored by the Research Grants Council of Hong Kong and the Consulate General of France in Hong Kong, F-HK010/127 and Institute of Molecular Functional Materials which was supported by a grant from the University Grants Committee, Areas of Excellence Scheme (AoE/P-03/08). This work was also supported by CityU Strategic Research Grant 9616302 and 7004026, and National Science Foundation of China 21324077. Publisher copyright: © 2015 American Chemical Society

PY - 2015/5/15

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N2 - We have demonstrated that the subcellular targeting properties of the indole-based cyanines can be tuned by the functional substituent attached onto the indole moiety in which the first example of a highly RNA-selective and two-photon active fluorescent light-up probe for high contrast and brightness TPEF images of rRNA in the nucleolus of live cells has been developed. It is important to find that this cyanine binds much stronger toward RNA than DNA in a buffer solution as well as selectively stains and targets to rRNA in the nucleolus. Remarkably, the TPEF brightness (Φσmax) is dramatically increased with 11-fold enhancement in the presence of rRNA, leading to the record high Φσmax of 228 GM for RNA. This probe not only shows good biocompatibility and superior photostability but also offers general applicability to various live cell lines including HeLa, HepG2, MCF-7, and KB cells and excellent counterstaining compatibility with commercially available DNA or protein trackers.

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Indole-based Cyanine as a Nuclear RNA-Selective Two-Photon Fluorescent Probe for Live Cell Imaging

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