TY - JOUR
T1 - Indigo Naturalis Suppresses Colonic Oxidative Stress and Th1/Th17 Responses of DSS-Induced Colitis in Mice
AU - Xiao, Haitao
AU - Peng, Jiao
AU - Wen, Bo
AU - Hu, Dong dong
AU - Hu, Xiao peng
AU - Shen, Xiang chun
AU - Liu, Zhi gang
AU - He, Zhen dan
AU - Bian, Zhaoxiang
N1 - Funding Information:
https://orcid.org/0000-0001-5951-4426 Xiao Hai-tao xhaitao@szu.edu.cn 1 2 3 Peng Jiao pengjiao153@163.com 4 Wen Bo 577189104@qq.com 2 3 Hu Dong-dong hdd1206@126.com 2 Hu Xiao-peng hu54398@2008.sina.com 1 Shen Xiang-chun sxc@gmc.edu.cn 3 Liu Zhi-gang lzg@szu.edu.cn 1 https://orcid.org/0000-0001-6876-3037 He Zhen-dan hezhendan@szu.edu.cn 1 https://orcid.org/0000-0001-6206-1958 Bian Zhao-xiang bianzxiang@gmail.com 2 5 Peluso Ilaria 1 School of Pharmaceutical Sciences Guangdong Key Laboratory for Genome Stability & Human Disease Prevention Shenzhen Key Laboratory of Novel Natural Health Care Products Innovation Platform for Natural Small Molecule Drugs Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs Health Science Center Shenzhen University Shenzhen 518060 China szu.edu.cn 2 School of Chinese Medicine Hong Kong Baptist University Kowloon Hong Kong hkbu.edu.hk 3 The Key Laboratory of Pharmacology and Druggability for Natural Medicines Department of Education Guizhou Medical University Guiyang Guizhou 550025 China gmc.edu.cn 4 Department of Pharmacy Peking University Shenzhen Hospital Shenzhen 518036 China pkuszh.com 5 Shenzhen Research Institute and Continuing Education Hong Kong Baptist University Shenzhen 518060 China hkbu.edu.hk 2019 13 10 2019 2019 21 02 2019 04 05 2019 25 06 2019 13 10 2019 2019 Copyright © 2019 Hai-tao Xiao et al. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Indigo naturalis (also known as Qing-dai, or QD), a traditional Chinese medicine, has been widely used as an anticolitis regimen in the clinical practice of Chinese medicine. However, the precise mechanisms behind its efficacy remain unknown. We investigated the protective effects and associated molecular mechanisms of QD in DSS-induced colitis in mice. We found that QD administration attenuated DSS-induced colon shortening, tissue damage, and the disease activity index during the onset of colitis. Moreover, QD administration significantly suppressed colonic MPO activity and increased the activities of colonic T-SOD, CAT, and GSH-Px, as well the expression of p-AMPK and Nrf-2 in colon tissues of colitic mice. In addition, QD was capable of reducing the colonic Th1 and Th17 cell cytokines, the frequencies of Th1 and Th17 cells, and the phosphorylation of p-STAT1 and p-STAT3 in the mesenteric lymph nodes of colitic mice. An in vitro assay showed that QD significantly suppressed the differentiation of Th1 and Th17 cells. These findings suggest that QD has the potential to alleviate experimental colitis by suppressing colonic oxidative stress and restraining colonic Th1/Th17 responses, which are associated with activating AMPK/Nrf-2 signals and inhibiting STAT1/STAT3 signals, respectively. These findings also support QD as an effective regimen in the treatment of IBD. National Key R&D Program of China 2017YFA053900 Shenzhen Science and Technology Innovation Commission JCYJ20170413170320959 Shenzhen University 2018020 Peacock Program for Overseas High-Level Talents of Shenzhen National Natural Science Foundation of China U1702286 31670360 81560676
PY - 2019/10/13
Y1 - 2019/10/13
N2 - Indigo naturalis (also known as Qing-dai, or QD), a traditional Chinese medicine, has been widely used as an anticolitis regimen in the clinical practice of Chinese medicine. However, the precise mechanisms behind its efficacy remain unknown. We investigated the protective effects and associated molecular mechanisms of QD in DSS-induced colitis in mice. We found that QD administration attenuated DSS-induced colon shortening, tissue damage, and the disease activity index during the onset of colitis. Moreover, QD administration significantly suppressed colonic MPO activity and increased the activities of colonic T-SOD, CAT, and GSH-Px, as well the expression of p-AMPK and Nrf-2 in colon tissues of colitic mice. In addition, QD was capable of reducing the colonic Th1 and Th17 cell cytokines, the frequencies of Th1 and Th17 cells, and the phosphorylation of p-STAT1 and p-STAT3 in the mesenteric lymph nodes of colitic mice. An in vitro assay showed that QD significantly suppressed the differentiation of Th1 and Th17 cells. These findings suggest that QD has the potential to alleviate experimental colitis by suppressing colonic oxidative stress and restraining colonic Th1/Th17 responses, which are associated with activating AMPK/Nrf-2 signals and inhibiting STAT1/STAT3 signals, respectively. These findings also support QD as an effective regimen in the treatment of IBD.
AB - Indigo naturalis (also known as Qing-dai, or QD), a traditional Chinese medicine, has been widely used as an anticolitis regimen in the clinical practice of Chinese medicine. However, the precise mechanisms behind its efficacy remain unknown. We investigated the protective effects and associated molecular mechanisms of QD in DSS-induced colitis in mice. We found that QD administration attenuated DSS-induced colon shortening, tissue damage, and the disease activity index during the onset of colitis. Moreover, QD administration significantly suppressed colonic MPO activity and increased the activities of colonic T-SOD, CAT, and GSH-Px, as well the expression of p-AMPK and Nrf-2 in colon tissues of colitic mice. In addition, QD was capable of reducing the colonic Th1 and Th17 cell cytokines, the frequencies of Th1 and Th17 cells, and the phosphorylation of p-STAT1 and p-STAT3 in the mesenteric lymph nodes of colitic mice. An in vitro assay showed that QD significantly suppressed the differentiation of Th1 and Th17 cells. These findings suggest that QD has the potential to alleviate experimental colitis by suppressing colonic oxidative stress and restraining colonic Th1/Th17 responses, which are associated with activating AMPK/Nrf-2 signals and inhibiting STAT1/STAT3 signals, respectively. These findings also support QD as an effective regimen in the treatment of IBD.
UR - http://www.scopus.com/inward/record.url?scp=85074295106&partnerID=8YFLogxK
U2 - 10.1155/2019/9480945
DO - 10.1155/2019/9480945
M3 - Article
C2 - 31737179
AN - SCOPUS:85074295106
SN - 1942-0900
VL - 2019
JO - Oxidative Medicine and Cellular Longevity
JF - Oxidative Medicine and Cellular Longevity
M1 - 9480945
ER -