Increased efficacies in magnetofection and gene delivery to hepatocellular carcinoma cells with ternary organic-inorganic hybrid nanocomposites

Ken Cham Fai Leung; Chun Pong Chak; Siu Fung Lee; Josie M.Y. Lai; Xiao Ming Zhu; Yi Xiang J. Wang; Kathy W.Y. Sham; Chi Hin Wong; Christopher H.K. Cheng

doi:10.1002/asia.201300202

Increased efficacies in magnetofection and gene delivery to hepatocellular carcinoma cells with ternary organic-inorganic hybrid nanocomposites

Ken Cham Fai Leung^*
, Chun Pong Chak
, Siu Fung Lee
, Josie M.Y. Lai
, Xiao Ming Zhu
, Yi Xiang J. Wang
, Kathy W.Y. Sham
, Chi Hin Wong
, Christopher H.K. Cheng

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

10 Citations (Scopus)

Abstract

A magnetic personality: Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic-inorganic hybrid nanocomposites that contain deferoxamine-coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellular uptake efficacies depend on the ratio of components in PEI/DNA/NP, as assessed by Prussian staining, magnetic resonance imaging, and luciferase expression in the presence and absence of a magnet during transfection.

Original language	English
Pages (from-to)	1760-1764
Number of pages	5
Journal	Chemistry - An Asian Journal
Volume	8
Issue number	8
DOIs	https://doi.org/10.1002/asia.201300202
Publication status	Published - Aug 2013

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

gene delivery
imaging agents
magnetofection
nanoparticles
organic-inorganic hybrid composites

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10.1002/asia.201300202

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Leung, K. C. F., Chak, C. P., Lee, S. F., Lai, J. M. Y., Zhu, X. M., Wang, Y. X. J., Sham, K. W. Y., Wong, C. H., & Cheng, C. H. K. (2013). Increased efficacies in magnetofection and gene delivery to hepatocellular carcinoma cells with ternary organic-inorganic hybrid nanocomposites. Chemistry - An Asian Journal, 8(8), 1760-1764. https://doi.org/10.1002/asia.201300202

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title = "Increased efficacies in magnetofection and gene delivery to hepatocellular carcinoma cells with ternary organic-inorganic hybrid nanocomposites",

abstract = "A magnetic personality: Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic-inorganic hybrid nanocomposites that contain deferoxamine-coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellular uptake efficacies depend on the ratio of components in PEI/DNA/NP, as assessed by Prussian staining, magnetic resonance imaging, and luciferase expression in the presence and absence of a magnet during transfection.",

keywords = "gene delivery, imaging agents, magnetofection, nanoparticles, organic-inorganic hybrid composites",

author = "Leung, \{Ken Cham Fai\} and Chak, \{Chun Pong\} and Lee, \{Siu Fung\} and Lai, \{Josie M.Y.\} and Zhu, \{Xiao Ming\} and Wang, \{Yi Xiang J.\} and Sham, \{Kathy W.Y.\} and Wong, \{Chi Hin\} and Cheng, \{Christopher H.K.\}",

year = "2013",

month = aug,

doi = "10.1002/asia.201300202",

language = "English",

volume = "8",

pages = "1760--1764",

journal = "Chemistry - An Asian Journal",

issn = "1861-4728",

publisher = "John Wiley \& Sons Ltd",

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AU - Leung, Ken Cham Fai

AU - Chak, Chun Pong

AU - Lee, Siu Fung

AU - Lai, Josie M.Y.

AU - Zhu, Xiao Ming

AU - Wang, Yi Xiang J.

AU - Sham, Kathy W.Y.

AU - Wong, Chi Hin

AU - Cheng, Christopher H.K.

PY - 2013/8

Y1 - 2013/8

N2 - A magnetic personality: Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic-inorganic hybrid nanocomposites that contain deferoxamine-coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellular uptake efficacies depend on the ratio of components in PEI/DNA/NP, as assessed by Prussian staining, magnetic resonance imaging, and luciferase expression in the presence and absence of a magnet during transfection.

AB - A magnetic personality: Magnetofection and gene delivery have been investigated in HepG2 cells using ternary organic-inorganic hybrid nanocomposites that contain deferoxamine-coated iron oxide nanoparticles (NPs), circular plasmid DNA, and branched polyethylenimine (PEI). It was found that the cellular uptake efficacies depend on the ratio of components in PEI/DNA/NP, as assessed by Prussian staining, magnetic resonance imaging, and luciferase expression in the presence and absence of a magnet during transfection.

KW - gene delivery

KW - imaging agents

KW - magnetofection

KW - nanoparticles

KW - organic-inorganic hybrid composites

UR - https://www.scopus.com/pages/publications/84881164643

U2 - 10.1002/asia.201300202

DO - 10.1002/asia.201300202

M3 - Journal article

C2 - 23729359

AN - SCOPUS:84881164643

SN - 1861-4728

VL - 8

SP - 1760

EP - 1764

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

IS - 8

ER -

Increased efficacies in magnetofection and gene delivery to hepatocellular carcinoma cells with ternary organic-inorganic hybrid nanocomposites

Abstract

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