@inbook{72a5d45eaac44ea4bf4fbc514372eb2f,
title = "In Vitro Reconstitution of Plant COPII Vesicles",
abstract = "In vitro reconstitution studies enable the controllable and stepwise investigation of complicated biochemical processes. In yeast and mammals, in vitro reconstitution of COPII vesicles marked a pivotal point in characterizing the endoplasmic reticulum-to-Golgi anterograde trafficking route and revealed how vesicles mediate the selective and reliable transportation among topologically equivalent compartments. By providing the necessary physiological conditions in a cell-free environment, it enables the dissection of essential components required for the vesicle formation. To enrich and purify the small amount in vivo membrane-bounded compartments, it simplifies the evaluation of vesicle regulation by distinct external stimuli or upstream signals. Here, we describe the preparation of plant microsomes and cytosol for the reconstitution of plant COPII vesicles. Purified vesicles can be used for further biochemical or microscopical analyses.",
keywords = "Endomembrane trafficking, In vitro reconstitution, Plant COPII vesicle",
author = "Baiying Li and Liwen Jiang",
note = "This work was supported by grants from the Research Grants Council of Hong Kong (AoE/M-05/12, CUHK14106823, C4033-19E, C4002-20 W, C4002-670 21EF, C2003-22WF, C4014-23G, G-CUHK409/23, R4005-18, Senior Research Fellow Scheme SRFS2122-4S01 and CRS_CUHK405/23), the Chinese University of Hong Kong (CUHK) Research Committee, and CAS-Croucher Funding Scheme for Joint Laboratories to LJ; the Hong Kong Baptist University (HKBU) Research Committee to BL. Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
month = aug,
day = "8",
doi = "10.1007/978-1-0716-4059-3_10",
language = "English",
isbn = "9781071640586",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "111--119",
editor = "Liwen Jiang and Jinbo Shen and Caiji Gao and Xiangfeng Wang",
booktitle = "Plant Protein Secretion",
edition = "2nd",
}