In vitro imaging and Human Serum Albumin responsive dimeric lanthanide DO3A complex

Yuen On Fung; Wanqing Wu; Chi Tung Yeung; Hoi Kuan Kong; Kenny Kam Cheng Wong; Wai Sum Lo; Ga Lai Law; Ka-Leung WONG; Chi Kong Lau; Chi Sing LEE; Wing Tak Wong

doi:10.1021/ic2004672

In vitro imaging and Human Serum Albumin responsive dimeric lanthanide DO3A complex

Yuen On Fung, Wanqing Wu, Chi Tung Yeung, Hoi Kuan Kong, Kenny Kam Cheng Wong, Wai Sum Lo, Ga Lai Law, Ka-Leung WONG, Chi Kong Lau, Chi Sing LEE, Wing Tak Wong^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

23 Citations (Scopus)

Abstract

Two series of dimeric DO3A (1,4,7,10-tetraazacyclodecane-1,4,7-triacetate) lanthanide complexes (LnL₁-LnL₂, Ln = Eu, Gd, and Tb) have been synthesized with two different bridged chromophores. The X-ray structures of dimeric LnL₁ (Ln = Gd and Tb) complexes show that each metal ion has nine coordination numbers with eight directly bound donor atoms of the ligand and one oxygen donor from the water molecule. Photophysical measurements indicate that the bridged antenna in LnL₂ gives a higher efficiency than that of LnL₁ and is responsive to the protein Human Serum Albumin (HSA), giving an f-f luminescence signal enhancement with a binding constant log K = 4.84. In vitro imaging of EuL₁ and EuL₂ in HeLa cells has been recorded, and EuL₂ has demonstrated a higher rate of cellular uptake and low cytotoxicity (IC₅₀ = 3 mM).

Original language	English
Pages (from-to)	5517-5525
Number of pages	9
Journal	Inorganic Chemistry
Volume	50
Issue number	12
DOIs	https://doi.org/10.1021/ic2004672
Publication status	Published - 20 Jun 2011

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Physical and Theoretical Chemistry
Inorganic Chemistry

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@article{73edcbb9445a42a4a0b446f7fc37db7e,

title = "In vitro imaging and Human Serum Albumin responsive dimeric lanthanide DO3A complex",

abstract = "Two series of dimeric DO3A (1,4,7,10-tetraazacyclodecane-1,4,7-triacetate) lanthanide complexes (LnL1-LnL2, Ln = Eu, Gd, and Tb) have been synthesized with two different bridged chromophores. The X-ray structures of dimeric LnL1 (Ln = Gd and Tb) complexes show that each metal ion has nine coordination numbers with eight directly bound donor atoms of the ligand and one oxygen donor from the water molecule. Photophysical measurements indicate that the bridged antenna in LnL2 gives a higher efficiency than that of LnL1 and is responsive to the protein Human Serum Albumin (HSA), giving an f-f luminescence signal enhancement with a binding constant log K = 4.84. In vitro imaging of EuL1 and EuL2 in HeLa cells has been recorded, and EuL2 has demonstrated a higher rate of cellular uptake and low cytotoxicity (IC50 = 3 mM).",

author = "Fung, {Yuen On} and Wanqing Wu and Yeung, {Chi Tung} and Kong, {Hoi Kuan} and Wong, {Kenny Kam Cheng} and Lo, {Wai Sum} and Law, {Ga Lai} and Ka-Leung WONG and Lau, {Chi Kong} and LEE, {Chi Sing} and Wong, {Wing Tak}",

note = "This work was funded by grants from The Hong Kong Research Grants Council (PolyU 503510P) and The Hong Kong Polytechnic University (1BD07), Hong Kong Chinese University, and Hong Kong Baptist University (FRG 1/10-11/ 037). This work was also supported by the Area of Excellence Scheme of the University of Grants Committee (Hong Kong). ",

year = "2011",

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doi = "10.1021/ic2004672",

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volume = "50",

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T1 - In vitro imaging and Human Serum Albumin responsive dimeric lanthanide DO3A complex

AU - Fung, Yuen On

AU - Wu, Wanqing

AU - Yeung, Chi Tung

AU - Kong, Hoi Kuan

AU - Wong, Kenny Kam Cheng

AU - Lo, Wai Sum

AU - Law, Ga Lai

AU - WONG, Ka-Leung

AU - Lau, Chi Kong

AU - LEE, Chi Sing

AU - Wong, Wing Tak

N1 - This work was funded by grants from The Hong Kong Research Grants Council (PolyU 503510P) and The Hong Kong Polytechnic University (1BD07), Hong Kong Chinese University, and Hong Kong Baptist University (FRG 1/10-11/ 037). This work was also supported by the Area of Excellence Scheme of the University of Grants Committee (Hong Kong).

PY - 2011/6/20

Y1 - 2011/6/20

N2 - Two series of dimeric DO3A (1,4,7,10-tetraazacyclodecane-1,4,7-triacetate) lanthanide complexes (LnL1-LnL2, Ln = Eu, Gd, and Tb) have been synthesized with two different bridged chromophores. The X-ray structures of dimeric LnL1 (Ln = Gd and Tb) complexes show that each metal ion has nine coordination numbers with eight directly bound donor atoms of the ligand and one oxygen donor from the water molecule. Photophysical measurements indicate that the bridged antenna in LnL2 gives a higher efficiency than that of LnL1 and is responsive to the protein Human Serum Albumin (HSA), giving an f-f luminescence signal enhancement with a binding constant log K = 4.84. In vitro imaging of EuL1 and EuL2 in HeLa cells has been recorded, and EuL2 has demonstrated a higher rate of cellular uptake and low cytotoxicity (IC50 = 3 mM).

AB - Two series of dimeric DO3A (1,4,7,10-tetraazacyclodecane-1,4,7-triacetate) lanthanide complexes (LnL1-LnL2, Ln = Eu, Gd, and Tb) have been synthesized with two different bridged chromophores. The X-ray structures of dimeric LnL1 (Ln = Gd and Tb) complexes show that each metal ion has nine coordination numbers with eight directly bound donor atoms of the ligand and one oxygen donor from the water molecule. Photophysical measurements indicate that the bridged antenna in LnL2 gives a higher efficiency than that of LnL1 and is responsive to the protein Human Serum Albumin (HSA), giving an f-f luminescence signal enhancement with a binding constant log K = 4.84. In vitro imaging of EuL1 and EuL2 in HeLa cells has been recorded, and EuL2 has demonstrated a higher rate of cellular uptake and low cytotoxicity (IC50 = 3 mM).

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In vitro imaging and Human Serum Albumin responsive dimeric lanthanide DO3A complex

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