@inbook{344ded05ba7b4d75ab24aec343ebd9b1,
title = "In Situ Observation of Abscisic Acid Distribution in Major Crop Species by Immunofluorescence Labeling",
abstract = "Immunofluorescent approach uses fluorophore-conjugated antibodies to target molecules of interest for the observation of their distribution and microenvironment at cellular or tissue level. In connection with modern fluorescence microscopy, immunofluorescence has high sensitivity and specificity, resulting in a wide range of applications in animal studies. However, the protocols of immunofluorescence are seldom reported. To this end, we describe an optimized protocol for the detection of plant hormone abscisic acid (ABA) in monocot crop species, including maize and rice. By using immunofluorescence technique provided from this protocol, investigation of ABA distribution will continue to deeper our insights in crop developments and stress responses.",
keywords = "Abscisic acid, Fluorescence microscopy, Immunofluorescence, Oryza sativa, Tissue imaging, Zea mays",
author = "Chen, {Mo Xian} and Lu, {Chong Chong} and Jianhua Zhang and Liu, {Ying Gao}",
note = "Funding Information: This work was supported by the Science Technology and Innovation Committee of Shenzhen (GJHZ20190821160401654), the National Natural Science Foundation of China (NSFC32001452), the Natural Science Foundation of Jiangsu Province (SBK2020042924), and Hong Kong Research Grant Council (AoE/M-05/12, AoE/M-403/16, GRF CUHK14160516, 14177617, 12100318). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
month = feb,
day = "12",
doi = "10.1007/978-1-0716-2156-1_12",
language = "English",
isbn = "9781071621554",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "155--162",
editor = "Takuya Yoshida",
booktitle = "Abscisic Acid",
edition = "1st",
}