TY - JOUR
T1 - In situ localization of lipids on mouse kidney tissues with acute cadmium toxicity using atmospheric pressure-MALDI mass spectrometry imaging
AU - Zeng, Ting
AU - Zhang, Rong
AU - Chen, Yanyan
AU - Guo, Wenjing
AU - Wang, Jianing
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by the National Key Research and Development Program of China (2017YFC1600500), the Start-up Grant from Hong Kong Baptist University, SKLEBA Research Grant (SKLP_2021_P04) and the Shenzhen Science and Technology Innovation Commission (2021Szvup134). We would like to thank MassTech and ASPEC Technologies Limited, for instrument support, and Xiaokun (Niko) Duan of ASPEC for technical assistance.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/8/1
Y1 - 2022/8/1
N2 - Cadmium-induced nephrotoxicity has been one of the major concerns for public health over the past century. Lipid peroxidation is a principal mechanism in its pathological process. Atmospheric pressure-MALDI mass spectrometry imaging (AP-MALDI MSI) enables direct mapping of lipids in the biological tissue sections. Considering the spatial visualization of lipids on mouse kidney tissues with acute cadmium toxicity is lacking, this study dedicates to filling the gap by using AP-MALDI MSI. Of the tested matrices, the optimized matrix for labeling lipids was 2,5-dihydroxyacetophenone (DHAP). A set of lipids including phosphatidylcholines (PC), phosphatidylglycerol (PG), lysophosphatidylcholine (LPC), sphingomyelin (SM), phosphatidic acid (PA), triglyceride (TG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), etc. were identified and visualized. Accordingly, PC, PG, LPC, SM, PA and TG were down-regulated while PE and PI were up-regulated in the renal cortex or medulla regions in kidney tissues of the mouse with acute cadmium toxicity. Such in situ locations of lipids on mouse kidney tissues with acute cadmium toxicity could help discover tissue-specific nephrotoxic biomarkers and provide new insights into its renal toxicological mechanism.
AB - Cadmium-induced nephrotoxicity has been one of the major concerns for public health over the past century. Lipid peroxidation is a principal mechanism in its pathological process. Atmospheric pressure-MALDI mass spectrometry imaging (AP-MALDI MSI) enables direct mapping of lipids in the biological tissue sections. Considering the spatial visualization of lipids on mouse kidney tissues with acute cadmium toxicity is lacking, this study dedicates to filling the gap by using AP-MALDI MSI. Of the tested matrices, the optimized matrix for labeling lipids was 2,5-dihydroxyacetophenone (DHAP). A set of lipids including phosphatidylcholines (PC), phosphatidylglycerol (PG), lysophosphatidylcholine (LPC), sphingomyelin (SM), phosphatidic acid (PA), triglyceride (TG), phosphatidylethanolamine (PE) and phosphatidylinositol (PI), etc. were identified and visualized. Accordingly, PC, PG, LPC, SM, PA and TG were down-regulated while PE and PI were up-regulated in the renal cortex or medulla regions in kidney tissues of the mouse with acute cadmium toxicity. Such in situ locations of lipids on mouse kidney tissues with acute cadmium toxicity could help discover tissue-specific nephrotoxic biomarkers and provide new insights into its renal toxicological mechanism.
KW - Acute toxicity
KW - AP-MALDI mass Spectrometry imaging
KW - Cadmium
KW - Lipids
KW - Mouse kidney
UR - http://www.scopus.com/inward/record.url?scp=85128670672&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2022.123466
DO - 10.1016/j.talanta.2022.123466
M3 - Journal article
SN - 0039-9140
VL - 245
JO - Talanta
JF - Talanta
M1 - 123466
ER -