Improved chromatographic fingerprints for facile differentiation of two Ganoderma spp

Chun Mei Fu, Guang Hua Lu, Oliver J. Schmitz, Zhang Wan Li, Kelvin S Y LEUNG*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

16 Citations (Scopus)


This paper addresses a comprehensive and comparative study of six phytochemical extraction methods for triterpenes from the fruiting body of Ganoderma spp. Quantitative analysis of extracts was performed by HPLC with photodiode array detection. In general, pressurized liquid extraction and microwave-assisted extraction under optimized conditions produce better yields, and the former also significantly reduces the total time of extraction and manipulation of a sample, as well as the amount of solvent used in comparison with conventional soxhlet, reflux, ultrasonic, and methanol-CO2 supercritical fluid extractions. Based on the improved extraction protocol, the fingerprinting profiles for two species of Lingzhi were established using the consistent chromatographic features of 12 authentic samples. Eleven common peaks of ganoderic/ganoderenic acids were identified using LC-ESI-MS-MS. These specific triterpene groups were adopted as chemical markers for Lingzhi. Using chemometric analysis, the developed fingerprinting was successfully applied to differentiate between the two species under the Ganoderma genus and is applicable as a method for quality evaluation of this valuable medicinal fungus and its related proprietary products.

Original languageEnglish
Pages (from-to)280-288
Number of pages9
JournalBiomedical Chromatography
Issue number3
Publication statusPublished - 2009

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

User-Defined Keywords

  • Extraction
  • Fingerprinting
  • Ganoderic acids
  • Ganoderma
  • HPLC
  • Triterpenes


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