Impairment of ftshi5 function affects cellular redox balance and photorespiratory metabolism in arabidopsis

Ting Wang, Sihui Li, Dan Chen, Yue Xi, Xuezhong Xu, Nenghui Ye, Jianhua ZHANG, Xinxiang Peng, Guohui Zhu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Photorespiration is an essential process for plant photosynthesis, development and growth in aerobic conditions. Recent studies have shown that photorespiration is an open system integrated with the plant primary metabolism network and intracellular redox systems, though the mechanisms of regulating photorespiration are far from clear. Through a forward genetic method, we identified a photorespiratory mutant pr1 (photorespiratory related 1), which produced a chlorotic and smaller photorespiratory growth phenotype with decreased chlorophyll content and accumulation of glycine and serine in ambient air. Morphological and physiological defects in pr1 plants can be largely abolished under elevated CO2 conditions. Genetic mapping and complementation confirmed that PR1 encodes an FtsH (Filamentation temperature-sensitive H)-like protein, FtsHi5. Reduced FtsHi5 expression in DEX-induced RNAi transgenic plants produced a similar growth phenotype with pr1 (ftsHi5-1). Transcriptome analysis suggested a changed expression pattern of redox-related genes and an increased expression of senescence-related genes in DEX: RNAi-FtsHi5 seedlings. Together with the observation that decreased accumulation of D1 and D2 proteins of photosystem II (PSII) and over-accumulation of reactive oxygen species (ROS) in ftsHi5 mutants, we hypothesize that FtsHi5 functions in maintaining the cellular redox balance and thus regulates photorespiratory metabolism.

Original languageEnglish
Pages (from-to)2526-2535
Number of pages10
JournalPlant and Cell Physiology
Volume59
Issue number12
DOIs
Publication statusPublished - 1 Dec 2018

Scopus Subject Areas

  • Physiology
  • Plant Science
  • Cell Biology

User-Defined Keywords

  • Arabidopsis
  • FtsHi5
  • Photorespiration
  • Redox balance

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