TY - JOUR
T1 - Impaired ossification coupled with accelerated cartilage degeneration in developmental dysplasia of the hip
T2 - Evidences from μcT arthrography in a rat model
AU - Fu, Ming
AU - LIU, Jin
AU - Huang, Guangxin
AU - Huang, Zhiyu
AU - Zhang, Zhiqi
AU - Wu, Peihui
AU - Wang, Bingjun
AU - Yang, Zibo
AU - Liao, Weiming
N1 - Funding Information:
These studies were supported by the National Natural Science Foundation of China (81171759; 81201388) and by China Science and Technology Grant of Guangdong (2011B031300012; 2009A03020001). We thank Prof. Huiling Yang from the Zhongshan School of Medicine, Sun Yat-sen University for her generous sharing of the bench for the experiments. We thank Prof. Ge Zhang and Dr. Baosheng Guo and Dr. Yixin He from the Hong Kong Baptist University for the generous supply of the Hexabrix 320 reagent and the valuable instruction on the CE-?CT imaging. We also thank Dr. Guoyan Liang from the Sun Yat-sen Memorial Hospital of Sun Yat-sen University for the helpful guidance on the histology analysis.
Funding Information:
These studies were supported by the National Natural Science Foundation of China (81171759; 81201388) and by China Science and Technology Grant of Guangdong (2011B031300012; 2009A03020001). We thank Prof. Huiling Yang from the Zhongshan School of Medicine, Sun Yat-sen University for her generous sharing of the bench for the experiments. We thank Prof. Ge Zhang and Dr. Baosheng Guo and Dr. Yixin He from the Hong Kong Baptist University for the generous supply of the Hexabrix 320 reagent and the valuable instruction on the CE-μCT imaging. We also thank Dr. Guoyan Liang from the Sun Yat-sen Memorial Hospital of Sun Yat-sen University for the helpful guidance on the histology analysis.
PY - 2014/10/8
Y1 - 2014/10/8
N2 - Background: Developmental dysplasia of the hip (DDH) always leads to cartilage degeneration and osteoarthritis of the hip joint. However, the diagnosis of early cartilage degeneration in DDH is still a clinical challenge. This study aims to investigate the dynamic changes of bone and cartilage in the hip of a rat model of DDH and to explore the potential application of microcomputed tomography (μCT) arthrography to detect early cartilage degeneration in DDH. Methods: Newborn Wistar rats were used to induce DDH by hindlimb swaddling. The bone and cartilage of the hip in model and control group were analyzed by μCT arthrography and histology examination at postnatal day 10, week 4, 6 and 8. Results: Hip dysplasia developed with age, became obvious at postnatal week 6 and further progressed at week 8. μCT analysis showed that bone mineral density (BMD) and bone volume density (bone volume over total volume, BV/TV) of the femoral head and neck region (FHNR) in model group were both significantly lower than those in control group, and they increased dramatically from postnatal week 4 to week 6 but maintained at a similar level at week 8. Contrast-enhanced μCT (CE-μCT) arthrography and histology data showed age-dependent increase in cartilage attenuation (CA) and decrease in safranin O staining intensity (SI) in model group, respectively. Moreover, the model group revealed remarkably higher CA and lower SI than control group, respectively. In addition, significant changes of CA and SI were both observed from postnatal week 6 to week 8 in model group. A strong linear correlation (r2 = 0.789, P <0.001) was found between CA and SI in model group. Furthermore, BMD was negatively correlated with SI (t = -2.683, P <0.05), whereas specific bone surface (bone surface over bone volume, BS/BV) was positively correlated with SI (t =4.501, P <0.01), in model group. Conclusions: Impaired ossification coupled with continuous loss of sGAG in cartilage matrix was found in the dysplasia hip during the disease progression of DDH. Cartilage degeneration in the dysplasia hip may occur early at childhood, accelerated with age and become irreversible at young adult stage. All these abnormal changes could be quantitatively assessed by μCT arthrography.
AB - Background: Developmental dysplasia of the hip (DDH) always leads to cartilage degeneration and osteoarthritis of the hip joint. However, the diagnosis of early cartilage degeneration in DDH is still a clinical challenge. This study aims to investigate the dynamic changes of bone and cartilage in the hip of a rat model of DDH and to explore the potential application of microcomputed tomography (μCT) arthrography to detect early cartilage degeneration in DDH. Methods: Newborn Wistar rats were used to induce DDH by hindlimb swaddling. The bone and cartilage of the hip in model and control group were analyzed by μCT arthrography and histology examination at postnatal day 10, week 4, 6 and 8. Results: Hip dysplasia developed with age, became obvious at postnatal week 6 and further progressed at week 8. μCT analysis showed that bone mineral density (BMD) and bone volume density (bone volume over total volume, BV/TV) of the femoral head and neck region (FHNR) in model group were both significantly lower than those in control group, and they increased dramatically from postnatal week 4 to week 6 but maintained at a similar level at week 8. Contrast-enhanced μCT (CE-μCT) arthrography and histology data showed age-dependent increase in cartilage attenuation (CA) and decrease in safranin O staining intensity (SI) in model group, respectively. Moreover, the model group revealed remarkably higher CA and lower SI than control group, respectively. In addition, significant changes of CA and SI were both observed from postnatal week 6 to week 8 in model group. A strong linear correlation (r2 = 0.789, P <0.001) was found between CA and SI in model group. Furthermore, BMD was negatively correlated with SI (t = -2.683, P <0.05), whereas specific bone surface (bone surface over bone volume, BS/BV) was positively correlated with SI (t =4.501, P <0.01), in model group. Conclusions: Impaired ossification coupled with continuous loss of sGAG in cartilage matrix was found in the dysplasia hip during the disease progression of DDH. Cartilage degeneration in the dysplasia hip may occur early at childhood, accelerated with age and become irreversible at young adult stage. All these abnormal changes could be quantitatively assessed by μCT arthrography.
KW - Cartilage degeneration
KW - CT arthrography
KW - Developmental dysplasia of the hip
KW - Impaired ossification
KW - μ
UR - http://www.scopus.com/inward/record.url?scp=84928808731&partnerID=8YFLogxK
U2 - 10.1186/1471-2474-15-339
DO - 10.1186/1471-2474-15-339
M3 - Journal article
C2 - 25294293
AN - SCOPUS:84928808731
SN - 1471-2474
VL - 15
JO - BMC Musculoskeletal Disorders
JF - BMC Musculoskeletal Disorders
IS - 1
M1 - 339
ER -