Impact of methyl jasmonate on squalene biosynthesis in microalga Schizochytrium mangrovei

Cai-Jun Yue, Yue Jiang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

45 Citations (Scopus)

Abstract

Squalene is an effective antioxidant and a potential chemopreventive agent. In this work, the effect of methyl jasmonate (MJA) on squalene biosynthesis in microalga Schizochytrium mangrovei was investigated. The maximum squalene content (1.17 ± 0.06 mg/g cell dry weight, DW) reached during the next 3 h after MJA treatment (0.1 mM) at 48 h of cultivation, which was 60% higher than that of control. The activity of squalene synthase (SS) increased 2-fold over control at this point. The maximum cholesterol content of 0.45 ± 0.03 mg/g DW was reached at hour 51 when MJA concentration was 0.4 mM, whereas the squalene content was lower at this point. The observations suggested that the increased squalene content was resulted from an increased activity of SS. MJA could be used to regulate the key enzymes in squalene biosynthetic pathway for the increased production of this compound in thraustochytrids. This research also provided novel information on the stimulation effect of methyl jasmonate on the biosynthesis of essential intermediate involved in the primary metabolism in microorganism.

Original languageEnglish
Pages (from-to)923-927
Number of pages5
JournalProcess Biochemistry
Volume44
Issue number8
DOIs
Publication statusPublished - Aug 2009
Externally publishedYes

Scopus Subject Areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

User-Defined Keywords

  • Cholesterol
  • Methyl jasmonate
  • Schizochytrium mangrovei
  • Squalene
  • Squalene synthase

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