Immunolesioning of nerve growth factor p75 receptor-containing neurons in the rat brain by a novel immunotoxin: Anti-p75-anti-mouse IgG-trichosanthin conjugates

K. H.H. Kwok, K. B. Law, Ricky N S WONG, Kin Lam YUNG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

In the present study, a comparison of potency between a commercially available immunotoxin, 192-immunoglobulin-SAP (192-IgG), and a novel immunotoxin produced in our laboratory, anti-p75-anti-mouse IgG-trichosanthin conjugates (p75-TCS), was conducted. Both of the immunotoxins were specific for nerve growth factor p75 receptor. Cholinergic neurons in the rat basal forebrain and in the neostriatum were depleted after the injection of either 192-IgG or p75-TCS. These indicate that both types of immunotoxins are potent and useful in performing immunolesioning experiments. In addition, there were variations in potency among the two immunotoxins in different routes of administration. The 192-IgG was more potent than the p75-TCS in the case of ventricular injections. In case of striatal injections, 192-IgG caused serious tissue necrosis and considerable tissue damage in the brain region. In contrast, p75-TCS was potent and caused a selective and specific depletion of cholinergic neurons in the neostriatum. These results indicate that indirect immunotoxins may be more useful for performing immunolesioning experiments in case of brain parenchyma administration. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)154-163
Number of pages10
JournalBrain Research
Volume846
Issue number2
DOIs
Publication statusPublished - 6 Nov 1999

Scopus Subject Areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

User-Defined Keywords

  • Basal forebrain
  • Cholinergic neuron
  • Immunocytochemistry
  • Neostriatum
  • Ribosome inactivating protein

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