TY - JOUR
T1 - Immunocytochemical localization of the metabotropic glutamate receptor mGluR4a in the piriform cortex of the rat
AU - Benítez, Rocío
AU - Fernández-Capetillo, Oscar
AU - Lázaro, Esther
AU - Mateos, José María
AU - Osorio, Alexandra
AU - Elezgarai, Izaskun
AU - Bilbao, Aurora
AU - Lingenhoehl, Kurt
AU - Van Der Putten, Herman
AU - Hampson, David R.
AU - Kuhn, Rainer
AU - Knöpfel, Thomas
AU - Grandes, Pedro
N1 - Research funding
Ministerio de Educación y Cultura, DGICYT. Grant Number: PB95-0347
Gobierno Vasco. Grant Numbers: PI-1997-41, BFI 95.154, BFI97.049, BFI98.109, BFI98.18
The Basque Country University
Publisher Copyright:
Copyright © 2000 Wiley-Liss, Inc.
PY - 2000/2/14
Y1 - 2000/2/14
N2 - This study evaluates the localization of the metabotropic glutamate receptor mGluR4a in the piriform cortex of rats using preembedding immunocytochemical methods. At the light microscopic level, punctate labeling was evident in layers Ia and Ib of the piriform cortex, and immunolabeled fibers were present in layers II and III. Following bilateral destruction of the olfactory bulb, the density of labeled puncta in layer Ia decreased. These results suggest that the receptor is present on the terminals of the lateral olfactory tract (LOT). Electron microscopic evaluation of layers Ia and lb revealed that mGluR4a was localized in synaptic terminals in layers Ia and Ib. The terminals had clear, round synaptic vesicles and terminated on asymmetric synapses on dendritic spines and shafts. There was also immunolabeling of some dendritic profiles in layers Ia and Ib that were postsynaptic to unlabeled presynaptic terminals. These observations suggest that mGluR4a is present on presynaptic terminals in the layers of the piriform cortex that receive LOT and associational synapses. This is the same area in which previous studies have revealed the presence of mGluR7 and mGluR8, suggesting that all three receptors may be colocalized. (C) Wiley- Liss, Inc.
AB - This study evaluates the localization of the metabotropic glutamate receptor mGluR4a in the piriform cortex of rats using preembedding immunocytochemical methods. At the light microscopic level, punctate labeling was evident in layers Ia and Ib of the piriform cortex, and immunolabeled fibers were present in layers II and III. Following bilateral destruction of the olfactory bulb, the density of labeled puncta in layer Ia decreased. These results suggest that the receptor is present on the terminals of the lateral olfactory tract (LOT). Electron microscopic evaluation of layers Ia and lb revealed that mGluR4a was localized in synaptic terminals in layers Ia and Ib. The terminals had clear, round synaptic vesicles and terminated on asymmetric synapses on dendritic spines and shafts. There was also immunolabeling of some dendritic profiles in layers Ia and Ib that were postsynaptic to unlabeled presynaptic terminals. These observations suggest that mGluR4a is present on presynaptic terminals in the layers of the piriform cortex that receive LOT and associational synapses. This is the same area in which previous studies have revealed the presence of mGluR7 and mGluR8, suggesting that all three receptors may be colocalized. (C) Wiley- Liss, Inc.
KW - Excitatory amino acid receptor
KW - G proteins
KW - Immunocytochemistry
KW - Lateral olfactory tract
KW - Olfactory system
UR - http://www.scopus.com/inward/record.url?scp=0342680163&partnerID=8YFLogxK
UR - https://onlinelibrary.wiley.com/doi/10.1002/(SICI)1096-9861(20000214)417:3%3C263::AID-CNE1%3E3.0.CO;2-H
U2 - 10.1002/(SICI)1096-9861(20000214)417:3<263::AID-CNE1>3.0.CO;2-H
DO - 10.1002/(SICI)1096-9861(20000214)417:3<263::AID-CNE1>3.0.CO;2-H
M3 - Journal article
C2 - 10683602
AN - SCOPUS:0342680163
SN - 0021-9967
VL - 417
SP - 263
EP - 274
JO - Journal of Comparative Neurology
JF - Journal of Comparative Neurology
IS - 3
ER -
