Immobilization of beef liver catalase on eggshell membrane for fabrication of hydrogen peroxide biosensor

Martin M F CHOI*, Tak Pong Yiu

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

68 Citations (Scopus)


A simple and low-cost hydrogen peroxide biosensor was fabricated using a beef liver catalase-immobilized eggshell membrane and an oxygen electrode. Catalase from beef liver extract was covalently immobilized on an eggshell membrane and subsequently covered the surface of an oxygen electrode. The detection scheme was based on the increase in dissolved oxygen content upon exposure to hydrogen peroxide solution. The increase in oxygen level was then monitored and related to the hydrogen peroxide concentration. The effects of enzyme loading, dissolved oxygen content, pH, phosphate buffer concentration and temperature on the biosensor have been studied in detail. The response and recovery times of the biosensor (t95) were 1min, respectively. The detection limit based on 3σb was 3μM and the relative standard deviation of the response was 1.28% (n=10) for a solution containing 0.3mM of H2O2. The hydrogen peroxide biosensor demonstrated a reasonable long and stable shelf-life. It has been successfully applied to the determination of hydrogen peroxide concentration in some commercial samples.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalEnzyme and Microbial Technology
Issue number1
Publication statusPublished - 5 Jan 2004

Scopus Subject Areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

User-Defined Keywords

  • Catalase
  • Eggshell membrane
  • Hydrogen peroxide


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