Imaging of brain slices with a genetically encoded voltage indicator

Peter Quicke; Samuel J. Barnes; Thomas Knöpfel

doi:10.1007/978-1-4939-6810-7_5

Imaging of brain slices with a genetically encoded voltage indicator

Peter Quicke
, Samuel J. Barnes
, Thomas Knöpfel^*

^*Corresponding author for this work

Department of Physics

Research output: Chapter in book/report/conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.

Original language	English
Title of host publication	Light Microscopy
Subtitle of host publication	Methods and Protocols
Editors	Yolanda Markaki, Hartmann Harz
Publisher	Humana New York, NY
Chapter	5
Pages	73-84
Number of pages	12
Edition	1st
ISBN (Electronic)	9781493968107
ISBN (Print)	9781493968084, 9781493983056
DOIs	https://doi.org/10.1007/978-1-4939-6810-7_5
Publication status	Published - 21 Mar 2017

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	1563
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Brain slices
Fluorescence imaging
Fluorescence microscopy
Voltage imaging
Voltage indicators
Voltage sensitive fluorescent proteins

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10.1007/978-1-4939-6810-7_5

Cite this

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abstract = "Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.",

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Imaging of brain slices with a genetically encoded voltage indicator. / Quicke, Peter; Barnes, Samuel J.; Knöpfel, Thomas.
Light Microscopy: Methods and Protocols. ed. / Yolanda Markaki; Hartmann Harz. 1st. ed. Humana New York, NY, 2017. p. 73-84 (Methods in Molecular Biology; Vol. 1563).

Research output: Chapter in book/report/conference proceeding › Chapter › peer-review

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AB - Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.

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ER -

Imaging of brain slices with a genetically encoded voltage indicator

Abstract

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