@article{3643cf6d3b6f4b50b7d67fee7e49c4f0,
title = "Imaging and analysis on the interaction between human antigen-pulsed Vδ2 T cells and antigen-specific CD4 T cells",
abstract = "This protocol describes how to visualize surface protein-protein co-localization across a cell-cell interface between antigen-presenting γδ-T cells and CD4 T cells. By consolidating immunofluorescence assay, confocal microscopy and 3D imaging analysis, it enables assessment of interaction between cell surface proteins such as Δ42PD1 and TLR4 between co-cultured γδ-T and CD4 T cells. This protocol can be applied to study a surface protein of interest and its potential interaction with a target cell/protein at the cell-cell interface. For complete details on the use and execution of this profile, please refer to Mo et al. (2020).",
keywords = "Cell culture, Cell isolation, Immunology, Microscopy",
author = "Yufei Mo and Cheung, {Ka Loon Allen} and Yue Liu and Li Liu and Zhiwei Chen",
note = "Funding Information: We thank Jingjing Li and Dongyan Zhou for generating the anti-Δ42PD1 monoclonal antibodies that were used in this study. This work was supported by Hong Kong Research Grant Council TRS-T11-706/18-N , GRF17115818 , GRF17104919 , GRF17103514 , and HKU5/CRF/13G , University Development Fund of the University of Hong Kong to AIDS Institute and the National Science and Technology Major Project 2018ZX10731-101-002-001 (to Z.C.), and Health and Medical Research Fund ( HMRF ) ( 15140732 and 18170032 ), Interdisciplinary Research Matching Scheme ( RC-IRCs-1718-03 ), Faculty Research Grant ( FRG2/17-18/066 ), Faculty Start-up Fund ( SCI-17-18-01 ), Tier 2 Start-up Grant ( RC-SGT2/18-19/SCI/007 ) (to A.K.L.C.). ",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100453",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}