Imaging and analysis on the interaction between human antigen-pulsed Vδ2 T cells and antigen-specific CD4 T cells

Yufei Mo, Ka Loon Allen CHEUNG*, Yue Liu, Li Liu, Zhiwei Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol describes how to visualize surface protein-protein co-localization across a cell-cell interface between antigen-presenting γδ-T cells and CD4 T cells. By consolidating immunofluorescence assay, confocal microscopy and 3D imaging analysis, it enables assessment of interaction between cell surface proteins such as Δ42PD1 and TLR4 between co-cultured γδ-T and CD4 T cells. This protocol can be applied to study a surface protein of interest and its potential interaction with a target cell/protein at the cell-cell interface. For complete details on the use and execution of this profile, please refer to Mo et al. (2020).

Original languageEnglish
Article number100453
JournalSTAR Protocols
Volume2
Issue number2
DOIs
Publication statusPublished - 18 Jun 2021

Scopus Subject Areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

User-Defined Keywords

  • Cell culture
  • Cell isolation
  • Immunology
  • Microscopy

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