Identification of the major chemical constituents and their metabolites in rat plasma and various organs after oral administration of effective Erxian Decoction (EXD) fraction by liquid chromatography-mass spectrometry

Yong Mei Hu, Yi Tao Wang, Stephen C. W. Sze, K. W. Tsang, H. K. Wong, Qing Liu, Li Dan Zhong, Yao Tong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A simple and specific LC-DAD-ESI-MS/MS method has been developed and applied for the primary investigation of the chemical constituents absorbed or metabolized in vivo, after the rat oral administration of Erxian Decoction (EXD), a Chinese medicine prescription for menopausal syndromes. Through the online ESI-MSn analysis, a total of 35 compounds have been identified or tentatively characterized from the seven tested samples, and 13 of them were unambiguously identified through a direct comparison of the retention time, UV spectra and MSn fragmentation patterns with the authentic ones. The results showed that 21 compounds were detected from rat plasma, 20 compounds were detected from rat kidneys and adrenal glands, 19 compounds were detected from rat ovaries, 12 compounds were found in rat intestines, nine compounds were identified from rat livers and nine compounds were detected from rat brains at certain time points after oral administration of the effective EXD fraction. 
Original languageEnglish
Pages (from-to)479-489
Number of pages11
JournalBiomedical Chromatography
Volume24
Issue number5
Early online date17 Aug 2009
DOIs
Publication statusPublished - May 2010
Externally publishedYes

User-Defined Keywords

  • Erxian Decoction (EXD)
  • LC-DAD-ESI-MS
  • Menopausal syndromes
  • Metabolites
  • Pharmacokinetic study
  • Traditional Chinese medicine prescriptions (TCMP)

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