Identification of the botanical source of Stemonae Radix based on polymerase chain reaction with specific primers and polymerase chain reaction-restriction fragment length polymorphism

Lan Lan Fan, Shu Zhu, Hubiao CHEN, Dong Hui Yang, Shao Qing Cai*, Katsuko Komatsu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Stemona sessilifolia, S. japonica and S. tuberosa are the three genuine sources of Stemonae Radix specified in the Chinese Pharmacopoeia (CP) for antitussive and insecticidal remedy. Significant variations in alkaloids composition and content, as well as different degree of antitussive activity were found among them. In order to accurately identify the genuine sources of Stemonae Radix in the genetic level, two polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were developed based on the sequence differences in chloroplast DNA trnL-trnF and petB-petD regions of the species recorded in CP, as well as S. parviflora and a counterfeit of Stemonae Radix, Asparagus cochinchinensis. By using the restriction enzymes MwoI, AciI and XmnI which were able to recognize specific sequence sites in the trnL-trnF region, and BclI, HincII and BslI which can recognize those in the petB-petD region to digest the corresponding PCR products, the specific digestion pattern enabled the discrimination of the botanical sources of Stemonae Radix effectively and efficiently.

Original languageEnglish
Pages (from-to)1624-1627
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume32
Issue number9
DOIs
Publication statusPublished - Sep 2009

Scopus Subject Areas

  • Pharmacology
  • Pharmaceutical Science

User-Defined Keywords

  • Molecular identification
  • petB-petD
  • Polymerase chain reaction-restriction fragment length polymorphism
  • Stemona
  • Stemonae Radix
  • trnL-trnF

Fingerprint

Dive into the research topics of 'Identification of the botanical source of Stemonae Radix based on polymerase chain reaction with specific primers and polymerase chain reaction-restriction fragment length polymorphism'. Together they form a unique fingerprint.

Cite this