Identification of sedative-hypnotic compounds shared by five medicinal Polyporales mushrooms using UPLC-Q-TOF-MS/MS-based untargeted metabolomics

Wei Chen, Jun Wen Yu, Yu Yi Deng, Lut Yi Wong, Chen Wang, Yu Ling Liang, Yuk Tung Leung, Jia Yi Tian, Ying Wu, Kelvin Sze Yin Leung, Jinhui Hu, Wen Hua Chen, Xiaobing Dou, Xiu Qiong Fu, Ying Jie Chen*, Zhi Ling Yu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

Background: Five Polyporales mushrooms, namely Amauroderma rugosum, Ganoderma lucidum, G. resinaceum, G. sinense and Trametes versicolor, are commonly used in China for managing insomnia. However, their active components for this application are not fully understood, restricting their universal recognition.

Purpose: In this study, we aimed to identify sedative-hypnotic compounds shared by these five Polyporales mushrooms. 

Study design and Methods: A UPLC-Q-TOF-MS/MS-based untargeted metabolomics, including OPLS-DA (orthogonal projection of potential structure discriminant analysis) and OPLS (orthogonal projections to latent structures) analysis together with mouse assays, were used to identify the main sedative-hypnotic compounds shared by the five Polyporales mushrooms. A pentobarbital sodium-induced sleeping model was used to investigate the sedative-hypnotic effects of the five mushrooms and their sedative-hypnotic compounds. 

Results: Ninety-two shared compounds in the five mushrooms were identified. Mouse assays showed that these mushrooms exerted sedative-hypnotic effects, with different potencies. Six triterpenes [four ganoderic acids (B, C1, F and H) and two ganoderenic acids (A and D)] were found to be the main sedative-hypnotic compounds shared by the five mushrooms. 

Conclusion: We for the first time found that these six triterpenes contribute to the sedative-hypnotic ability of the five mushrooms. Our novel findings provide pharmacological and chemical justifications for the use of the five medicinal mushrooms in managing insomnia.

Original languageEnglish
Article number155355
Number of pages11
JournalPhytomedicine
Volume128
DOIs
Publication statusPublished - 12 Jan 2024

Scopus Subject Areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

User-Defined Keywords

  • Medicinal Polyporales mushrooms
  • Sedative-hypnotic compounds
  • Untargeted metabolomics

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