TY - JOUR
T1 - Identification of immune-related genes in gill cells of Japanese eels (Anguilla japonica) in adaptation to water salinity changes
AU - Gu, Jie
AU - Dai, Shuya
AU - Liu, Haitao
AU - Cao, Quanquan
AU - Yin, Shaowu
AU - Lai, Keng Po
AU - TSE, William K F
AU - WONG, Chris K C
AU - Shi, Haifeng
N1 - Funding Information:
The work in Gu's laboratory is supported by the Start-Up Research Funding of Jiangsu University for Distinguished Scholars ( 5501330001 ) and the National Natural Science Foundation of China ( 31271272 and 31600952 ).
Funding Information:
The work in Gu's laboratory is supported by the Start-Up Research Funding of Jiangsu University for Distinguished Scholars (5501330001) and the National Natural Science Foundation of China (31271272 and 31600952).
PY - 2018/2
Y1 - 2018/2
N2 - The changes in ambient salinity influence ion and water homeostasis, hormones secretion, and immune response in fish gills. The physiological functions of hormones and ion transporters in the regulation of gill-osmoregulation have been widely studied, however the modulation of immune response under salinity changes is not determined. Using transcriptome sequencing, we obtained a comprehensive profile of osmo-responsive genes in gill cells of Japanese eel (Anguilla japonica). Herein, we applied bioinformatics analysis to identify the immune-related genes that were significantly higher expressed in gill pavement cells (PVCs) and mitochondrial-rich cells (MRCs) in freshwater (FW) than seawater (SW) adapted fish. We validated the data using the real-time qPCR, which showed a high correlation between the RNA-seq and real-time qPCR data. In addition, the immunohistochemistry results confirmed the changes of the expression of selected immune-related genes, including C-reactive protein (CRP) in PVCs, toll-like receptor 2 (TLR2) in MRCs and interleukin-1 receptor type 2 (IL-1R2) in both PVCs and MRCs. Collectively our results demonstrated that those immune-related genes respond to salinity changes, and might trigger related special signaling pathways and network. This study provides new insights into the impacts of ambient salinity changes on adaptive immune response in fish gill cells.
AB - The changes in ambient salinity influence ion and water homeostasis, hormones secretion, and immune response in fish gills. The physiological functions of hormones and ion transporters in the regulation of gill-osmoregulation have been widely studied, however the modulation of immune response under salinity changes is not determined. Using transcriptome sequencing, we obtained a comprehensive profile of osmo-responsive genes in gill cells of Japanese eel (Anguilla japonica). Herein, we applied bioinformatics analysis to identify the immune-related genes that were significantly higher expressed in gill pavement cells (PVCs) and mitochondrial-rich cells (MRCs) in freshwater (FW) than seawater (SW) adapted fish. We validated the data using the real-time qPCR, which showed a high correlation between the RNA-seq and real-time qPCR data. In addition, the immunohistochemistry results confirmed the changes of the expression of selected immune-related genes, including C-reactive protein (CRP) in PVCs, toll-like receptor 2 (TLR2) in MRCs and interleukin-1 receptor type 2 (IL-1R2) in both PVCs and MRCs. Collectively our results demonstrated that those immune-related genes respond to salinity changes, and might trigger related special signaling pathways and network. This study provides new insights into the impacts of ambient salinity changes on adaptive immune response in fish gill cells.
KW - Adaptive immune response
KW - Environmental salinity
KW - Immune-related genes
KW - RNA-seq
UR - http://www.scopus.com/inward/record.url?scp=85039171552&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2017.12.026
DO - 10.1016/j.fsi.2017.12.026
M3 - Journal article
C2 - 29269288
AN - SCOPUS:85039171552
SN - 1050-4648
VL - 73
SP - 288
EP - 296
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -