Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus

Ning Liu, Kim Chung Lee, Wenjun Song, Pui Wang, Zongwei CAI*, Honglin Chen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion of nucleoprotein of avian influenza virus (H5N1) and to identify three mutated peptides. The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively.

Original languageEnglish
Pages (from-to)1492-1496
Number of pages5
JournalTalanta
Volume78
Issue number4-5
DOIs
Publication statusPublished - 15 Jun 2009

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Amino acid substitution
  • H5N1 virus
  • Mutated peptide
  • Nanospray-MS/MS
  • Nucleoprotein

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