Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus

Ning Liu; Kim Chung Lee; Wenjun Song; Pui Wang; Zongwei CAI; Honglin Chen

doi:10.1016/j.talanta.2009.01.057

Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus

Ning Liu, Kim Chung Lee, Wenjun Song, Pui Wang, Zongwei CAI^*, Honglin Chen

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

4 Citations (Scopus)

Abstract

Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion of nucleoprotein of avian influenza virus (H5N1) and to identify three mutated peptides. The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively.

Original language	English
Pages (from-to)	1492-1496
Number of pages	5
Journal	Talanta
Volume	78
Issue number	4-5
DOIs	https://doi.org/10.1016/j.talanta.2009.01.057
Publication status	Published - 15 Jun 2009

Scopus Subject Areas

Analytical Chemistry

User-Defined Keywords

Amino acid substitution
H5N1 virus
Mutated peptide
Nanospray-MS/MS
Nucleoprotein

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10.1016/j.talanta.2009.01.057

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@article{d9ad11d5fa084a21b69c921dd5b6328b,

title = "Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus",

abstract = "Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion of nucleoprotein of avian influenza virus (H5N1) and to identify three mutated peptides. The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively.",

keywords = "Amino acid substitution, H5N1 virus, Mutated peptide, Nanospray-MS/MS, Nucleoprotein",

author = "Ning Liu and Lee, {Kim Chung} and Wenjun Song and Pui Wang and Zongwei CAI and Honglin Chen",

note = "Funding Information: The authors would like to acknowledge financial support from the Faculty Research Grant (FRG/07-08/11-21) from Hong Kong Baptist University. ",

year = "2009",

month = jun,

day = "15",

doi = "10.1016/j.talanta.2009.01.057",

language = "English",

volume = "78",

pages = "1492--1496",

journal = "Talanta",

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TY - JOUR

T1 - Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus

AU - Liu, Ning

AU - Lee, Kim Chung

AU - Song, Wenjun

AU - Wang, Pui

AU - CAI, Zongwei

AU - Chen, Honglin

N1 - Funding Information: The authors would like to acknowledge financial support from the Faculty Research Grant (FRG/07-08/11-21) from Hong Kong Baptist University.

PY - 2009/6/15

Y1 - 2009/6/15

N2 - Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion of nucleoprotein of avian influenza virus (H5N1) and to identify three mutated peptides. The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively.

AB - Nucleoprotein (NP), the structural component of ribonucleoprotein complex of avian influenza virus, performs multiple essential functions in the regulation of viral RNA synthesis and in the control of nuclear traffic of viral proteins. Mutations have often been found in NP, some of which are relevant to viral survival strategies. In this study, we used nanospray-MS/MS to analyze tryptic digestion of nucleoprotein of avian influenza virus (H5N1) and to identify three mutated peptides. The MS/MS analyses allowed the confident determination of the three mutated amino acid residues F313Y, I194V and V408I/L in the mutated peptides of LLQNSQVYSLIRPNENPAHK, GVGTMVMELVR and ASAGQI/LSVQPTFSVQR, respectively.

KW - Amino acid substitution

KW - H5N1 virus

KW - Mutated peptide

KW - Nanospray-MS/MS

KW - Nucleoprotein

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U2 - 10.1016/j.talanta.2009.01.057

DO - 10.1016/j.talanta.2009.01.057

M3 - Journal article

C2 - 19362222

AN - SCOPUS:63949083467

SN - 0039-9140

VL - 78

SP - 1492

EP - 1496

JO - Talanta

JF - Talanta

IS - 4-5

ER -

Identification of amino acid substitutions in mutated peptides of nucleoprotein from avian influenza virus

Abstract

Scopus Subject Areas

User-Defined Keywords

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