TY - JOUR
T1 - Identification of a mouse synaptic glycoprotein gene in cultured neurons
AU - Yu, Albert Cheung Hoi
AU - Sun, Chun Xiao
AU - Li, Qiang
AU - Liu, Hua Dong
AU - Wang, Chen Ran
AU - Zhao, Guo Ping
AU - Jin, Meilei
AU - Lau, Lok Ting
AU - Fung, Yin Wan Wendy
AU - Liu, Shuang
N1 - This study was supported by grants from the Shanghai Commission of Science & Technology Grant 99JC14024; Shanghai Research Center of Life Sciences, Chinese Academy of Sciences; Research Grant Council (H.K.) HKUST6177/97M; HKUST/CAS Joint Laboratory Scheme; the North American Medical Association Foundation (Hong Kong) NAMA 94/95.SC01; the Natural Science Foundation of China (30270426, 30470543) and the Beijing Natural Science Foundation (7032026, 7051004) to ACHY.
Publisher Copyright:
© 2005 Springer Science+Business Media, Inc.
PY - 2005/10
Y1 - 2005/10
N2 - Neuronal differentiation and aging are known to involve many genes, which may also be differentially expressed during these developmental processes. From primary cultured cerebral cortical neurons, we have previously identified various differentially expressed gene transcripts from cultured cortical neurons using the technique of arbitrarily primed PCR (RAP-PCR). Among these transcripts, clone 0-2 was found to have high homology to rat and human synaptic glycoprotein. By in silico analysis using an EST database and the FACTURA software, the full-length sequence of 0-2 was assembled and the clone was named as mouse synaptic glycoprotein homolog 2 (mSC2). DNA sequencing revealed transcript size of mSC2 being smaller than the human and rat homologs. RT-PCR indicated that mSC2 was expressed differentially at various culture days. The mSC2 gene was located in various tissues with higher expression in brain, lung, and liver. Functions of mSC2 in neurons and other tissues remain elusive and will require more investigation.
AB - Neuronal differentiation and aging are known to involve many genes, which may also be differentially expressed during these developmental processes. From primary cultured cerebral cortical neurons, we have previously identified various differentially expressed gene transcripts from cultured cortical neurons using the technique of arbitrarily primed PCR (RAP-PCR). Among these transcripts, clone 0-2 was found to have high homology to rat and human synaptic glycoprotein. By in silico analysis using an EST database and the FACTURA software, the full-length sequence of 0-2 was assembled and the clone was named as mouse synaptic glycoprotein homolog 2 (mSC2). DNA sequencing revealed transcript size of mSC2 being smaller than the human and rat homologs. RT-PCR indicated that mSC2 was expressed differentially at various culture days. The mSC2 gene was located in various tissues with higher expression in brain, lung, and liver. Functions of mSC2 in neurons and other tissues remain elusive and will require more investigation.
KW - Bioinformatics
KW - Cerebral cortical neurons
KW - Primary culture
KW - Synaptic glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=28844502412&partnerID=8YFLogxK
UR - https://link.springer.com/article/10.1007/s11064-005-8800-5
U2 - 10.1007/s11064-005-8800-5
DO - 10.1007/s11064-005-8800-5
M3 - Journal article
C2 - 16341590
AN - SCOPUS:28844502412
SN - 0364-3190
VL - 30
SP - 1289
EP - 1294
JO - Neurochemical Research
JF - Neurochemical Research
IS - 10
ER -
