Identification of α-glucosidase inhibitors from: Cyclocarya paliurus tea leaves using UF-UPLC-Q/TOF-MS/MS and molecular docking

Zi Wan Ning, Li Xiang Zhai, Tao Huang, Jiao Peng, Die Hu, Haitao XIAO*, Bo Wen, Cheng Yuan Lin, Ling Zhao, Zhaoxiang BIAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Leaves of Cyclocarya paliurus (CP) have a potential antihyperglycemic effect, but its active compositions responsible for the beneficial properties remain unclear. The CP extract exhibited remarkable α-glucosidase inhibitory activity with an IC 50 value of 31.5 ± 1.05 μg mL -1 , much lower than that of the positive control acarbose (IC 50 = 296.6 ± 1.06 μg mL -1 ). To identify the specific α-glucosidase inhibitors from the CP extract, affinity ultrafiltration coupled with ultra-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry (UF-UPLC-Q/TOF-MS/MS) was developed and 11 potential α-glucosidase inhibitors from CP extract were identified. In vitro α-glucosidase inhibitory assay verified that quercetin-3-O-glucuronide, kaempferol-3-O-rhamnoside, quercetin, kaempferol, asiatic acid and genistein were primarily responsible for the α-glucosidase inhibitory activity of the CP extract. Further, a hypoglycemia test also verified that these α-glucosidase inhibitors had the potential to reduce post-prandial hyperglycaemia in C57BL/6 mice. Moreover, the molecular docking study revealed that these identified α-glucosidase inhibitors more easily occupy the active sites of α-glucosidase than does the positive control acarbose. These findings suggest the CP tea leaves are the potential source of a hypoglycaemic agent.

Original languageEnglish
Pages (from-to)1893-1902
Number of pages10
JournalFood and Function
Volume10
Issue number4
DOIs
Publication statusPublished - Apr 2019

Scopus Subject Areas

  • Food Science

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