Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings

Juan Chen; Ni Na Zhang; Qing Pan; Xue Yuan Lin; Zhouping Shangguan; Jian Hua Zhang; Ge Hong Wei

doi:10.1186/s12870-020-02601-2

Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings

Juan Chen^*, Ni Na Zhang, Qing Pan, Xue Yuan Lin, Zhouping Shangguan, Jian Hua Zhang, Ge Hong Wei^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

16 Citations (Scopus)

Abstract

Background: Hydrogen sulphide (H2S) is involved in regulating physiological processes in plants. We investigated how H2S ameliorates iron (Fe) deficiency in soybean (Glycine max L.) seedlings. Multidisciplinary approaches including physiological, biochemical and molecular, and transcriptome methods were used to investigate the H2S role in regulating Fe availability in soybean seedlings.

Results: Our results showed that H2S completely prevented leaf interveinal chlorosis and caused an increase in soybean seedling biomass under Fe deficiency conditions. Moreover, H2S decreased the amount of root-bound apoplastic Fe and increased the Fe content in leaves and roots by regulating the ferric-chelate reductase (FCR) activities and Fe homeostasis- and sulphur metabolism-related gene expression levels, thereby promoting photosynthesis in soybean seedlings. In addition, H2S changed the plant hormone concentrations by modulating plant hormone-related gene expression abundances in soybean seedlings grown in Fe-deficient solution. Furthermore, organic acid biosynthesis and related genes expression also played a vital role in modulating the H2S-mediated alleviation of Fe deficiency in soybean seedlings.

Conclusion: Our results indicated that Fe deficiency was alleviated by H2S through enhancement of Fe acquisition and assimilation, thereby regulating plant hormones and organic acid synthesis in plants.

Original language	English
Article number	383
Journal	BMC Plant Biology
Volume	20
Issue number	1
Early online date	20 Aug 2020
DOIs	https://doi.org/10.1186/s12870-020-02601-2
Publication status	Published - Dec 2020

Scopus Subject Areas

Plant Science

User-Defined Keywords

Hydrogen sulphide (H2S)
Iron assimilation
Iron deficiency
Organic acid
Plant hormones
Soybean (Glycine max)

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10.1186/s12870-020-02601-2

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@article{2bc7234afe3c4dd0a41cd4a41bb5c04d,

title = "Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings",

abstract = "Background: Hydrogen sulphide (H2S) is involved in regulating physiological processes in plants. We investigated how H2S ameliorates iron (Fe) deficiency in soybean (Glycine max L.) seedlings. Multidisciplinary approaches including physiological, biochemical and molecular, and transcriptome methods were used to investigate the H2S role in regulating Fe availability in soybean seedlings. Results: Our results showed that H2S completely prevented leaf interveinal chlorosis and caused an increase in soybean seedling biomass under Fe deficiency conditions. Moreover, H2S decreased the amount of root-bound apoplastic Fe and increased the Fe content in leaves and roots by regulating the ferric-chelate reductase (FCR) activities and Fe homeostasis- and sulphur metabolism-related gene expression levels, thereby promoting photosynthesis in soybean seedlings. In addition, H2S changed the plant hormone concentrations by modulating plant hormone-related gene expression abundances in soybean seedlings grown in Fe-deficient solution. Furthermore, organic acid biosynthesis and related genes expression also played a vital role in modulating the H2S-mediated alleviation of Fe deficiency in soybean seedlings. Conclusion: Our results indicated that Fe deficiency was alleviated by H2S through enhancement of Fe acquisition and assimilation, thereby regulating plant hormones and organic acid synthesis in plants. ",

keywords = "Hydrogen sulphide (H2S), Iron assimilation, Iron deficiency, Organic acid, Plant hormones, Soybean (Glycine max)",

author = "Juan Chen and Zhang, {Ni Na} and Qing Pan and Lin, {Xue Yuan} and Zhouping Shangguan and Zhang, {Jian Hua} and Wei, {Ge Hong}",

note = "Funding Information: This study was financially supported by the Natural Science Foundation of China (NSFC) (31501822) and the Postdoctoral Science Foundation of China (2015 M580876 and 2016 T90948), and the Hong Kong Research Grant Council (14122415, 14160516, 14177617, AoE/M-05/12, AoE/M-403/16). The funding bodies were not involved in the design of the study, collection, analysis, and interpretation of data, and in writing the manuscript. Publisher Copyright: {\textcopyright} The Author(s). 2020 Open Access Publisher{\textquoteright}s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.",

year = "2020",

month = dec,

doi = "10.1186/s12870-020-02601-2",

language = "English",

volume = "20",

journal = "BMC Plant Biology",

issn = "1471-2229",

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TY - JOUR

T1 - Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings

AU - Chen, Juan

AU - Zhang, Ni Na

AU - Pan, Qing

AU - Lin, Xue Yuan

AU - Shangguan, Zhouping

AU - Zhang, Jian Hua

AU - Wei, Ge Hong

N1 - Funding Information: This study was financially supported by the Natural Science Foundation of China (NSFC) (31501822) and the Postdoctoral Science Foundation of China (2015 M580876 and 2016 T90948), and the Hong Kong Research Grant Council (14122415, 14160516, 14177617, AoE/M-05/12, AoE/M-403/16). The funding bodies were not involved in the design of the study, collection, analysis, and interpretation of data, and in writing the manuscript. Publisher Copyright: © The Author(s). 2020 Open Access Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

PY - 2020/12

Y1 - 2020/12

N2 - Background: Hydrogen sulphide (H2S) is involved in regulating physiological processes in plants. We investigated how H2S ameliorates iron (Fe) deficiency in soybean (Glycine max L.) seedlings. Multidisciplinary approaches including physiological, biochemical and molecular, and transcriptome methods were used to investigate the H2S role in regulating Fe availability in soybean seedlings. Results: Our results showed that H2S completely prevented leaf interveinal chlorosis and caused an increase in soybean seedling biomass under Fe deficiency conditions. Moreover, H2S decreased the amount of root-bound apoplastic Fe and increased the Fe content in leaves and roots by regulating the ferric-chelate reductase (FCR) activities and Fe homeostasis- and sulphur metabolism-related gene expression levels, thereby promoting photosynthesis in soybean seedlings. In addition, H2S changed the plant hormone concentrations by modulating plant hormone-related gene expression abundances in soybean seedlings grown in Fe-deficient solution. Furthermore, organic acid biosynthesis and related genes expression also played a vital role in modulating the H2S-mediated alleviation of Fe deficiency in soybean seedlings. Conclusion: Our results indicated that Fe deficiency was alleviated by H2S through enhancement of Fe acquisition and assimilation, thereby regulating plant hormones and organic acid synthesis in plants.

AB - Background: Hydrogen sulphide (H2S) is involved in regulating physiological processes in plants. We investigated how H2S ameliorates iron (Fe) deficiency in soybean (Glycine max L.) seedlings. Multidisciplinary approaches including physiological, biochemical and molecular, and transcriptome methods were used to investigate the H2S role in regulating Fe availability in soybean seedlings. Results: Our results showed that H2S completely prevented leaf interveinal chlorosis and caused an increase in soybean seedling biomass under Fe deficiency conditions. Moreover, H2S decreased the amount of root-bound apoplastic Fe and increased the Fe content in leaves and roots by regulating the ferric-chelate reductase (FCR) activities and Fe homeostasis- and sulphur metabolism-related gene expression levels, thereby promoting photosynthesis in soybean seedlings. In addition, H2S changed the plant hormone concentrations by modulating plant hormone-related gene expression abundances in soybean seedlings grown in Fe-deficient solution. Furthermore, organic acid biosynthesis and related genes expression also played a vital role in modulating the H2S-mediated alleviation of Fe deficiency in soybean seedlings. Conclusion: Our results indicated that Fe deficiency was alleviated by H2S through enhancement of Fe acquisition and assimilation, thereby regulating plant hormones and organic acid synthesis in plants.

KW - Hydrogen sulphide (H2S)

KW - Iron assimilation

KW - Iron deficiency

KW - Organic acid

KW - Plant hormones

KW - Soybean (Glycine max)

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U2 - 10.1186/s12870-020-02601-2

DO - 10.1186/s12870-020-02601-2

M3 - Journal article

C2 - 32819279

AN - SCOPUS:85089769556

SN - 1471-2229

VL - 20

JO - BMC Plant Biology

JF - BMC Plant Biology

IS - 1

M1 - 383

ER -

Hydrogen sulphide alleviates iron deficiency by promoting iron availability and plant hormone levels in Glycine max seedlings

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