Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359T under capnophilic lactic fermentation conditions

Nirakar PRADHAN; Laura Dipasquale; Giuliana D’Ippolito; Antonio Panico; Piet N.L. Lens; Giovanni Esposito; Angelo Fontana

doi:10.1016/j.ijhydene.2017.05.052

Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359^T under capnophilic lactic fermentation conditions

Nirakar PRADHAN, Laura Dipasquale, Giuliana D’Ippolito, Antonio Panico, Piet N.L. Lens, Giovanni Esposito, Angelo Fontana

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

22 Citations (Scopus)

Abstract

Thermotoga neapolitana is a hyperthermophilic eubacterium that produces hydrogen by sugar fermentation. A lab strain of T. neapolitana DSMZ 4359^T maintained in a CO₂-enriched atmosphere showed a stable increase of lactic acid production under capnophilic lactic fermentation (CLF) conditions. The genotypic comparison between the putative mutant (TN-CMut) and the original strain DSMZ 4359^T (WT4359) revealed 88.1 (±2.4)% DNA homology. RiboPrint® and MALDI-TOF mass analyses support a genetic differentiation beyond subspecies level. The phenotypic characterization indicated a high correlation between the two strains, except for the lactic acid production. Under identical operating conditions, the lab mutant produced significantly more lactic acid than the parent strain without impairing the hydrogen yield. The highest divergence between TN-CMut and WT4359 was observed for fermentation of glucose or lactose at 80 °C. Based on these results, we propose that the lab strain is a new subspecies of the genus Thermotoga that is named T. neapolitana subsp. capnolactica with regards to its improved feature to produce lactic acid under capnophilic conditions.

Original language	English
Pages (from-to)	16023-16030
Number of pages	8
Journal	International Journal of Hydrogen Energy
Volume	42
Issue number	25
DOIs	https://doi.org/10.1016/j.ijhydene.2017.05.052
Publication status	Published - Jun 2017

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PRADHAN, N., Dipasquale, L., D’Ippolito, G., Panico, A., Lens, P. N. L., Esposito, G., & Fontana, A. (2017). Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359^T under capnophilic lactic fermentation conditions. International Journal of Hydrogen Energy, 42(25), 16023-16030. https://doi.org/10.1016/j.ijhydene.2017.05.052

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title = "Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359T under capnophilic lactic fermentation conditions",

abstract = "Thermotoga neapolitana is a hyperthermophilic eubacterium that produces hydrogen by sugar fermentation. A lab strain of T. neapolitana DSMZ 4359T maintained in a CO2-enriched atmosphere showed a stable increase of lactic acid production under capnophilic lactic fermentation (CLF) conditions. The genotypic comparison between the putative mutant (TN-CMut) and the original strain DSMZ 4359T (WT4359) revealed 88.1 (±2.4)% DNA homology. RiboPrint{\textregistered} and MALDI-TOF mass analyses support a genetic differentiation beyond subspecies level. The phenotypic characterization indicated a high correlation between the two strains, except for the lactic acid production. Under identical operating conditions, the lab mutant produced significantly more lactic acid than the parent strain without impairing the hydrogen yield. The highest divergence between TN-CMut and WT4359 was observed for fermentation of glucose or lactose at 80 °C. Based on these results, we propose that the lab strain is a new subspecies of the genus Thermotoga that is named T. neapolitana subsp. capnolactica with regards to its improved feature to produce lactic acid under capnophilic conditions.",

author = "Nirakar PRADHAN and Laura Dipasquale and Giuliana D{\textquoteright}Ippolito and Antonio Panico and Lens, {Piet N.L.} and Giovanni Esposito and Angelo Fontana",

year = "2017",

month = jun,

doi = "10.1016/j.ijhydene.2017.05.052",

language = "English",

volume = "42",

pages = "16023--16030",

journal = "International Journal of Hydrogen Energy",

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PRADHAN, N, Dipasquale, L, D’Ippolito, G, Panico, A, Lens, PNL, Esposito, G & Fontana, A 2017, 'Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359^T under capnophilic lactic fermentation conditions', International Journal of Hydrogen Energy, vol. 42, no. 25, pp. 16023-16030. https://doi.org/10.1016/j.ijhydene.2017.05.052

Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359^T under capnophilic lactic fermentation conditions. / PRADHAN, Nirakar; Dipasquale, Laura; D’Ippolito, Giuliana et al.
In: International Journal of Hydrogen Energy, Vol. 42, No. 25, 06.2017, p. 16023-16030.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359T under capnophilic lactic fermentation conditions

AU - PRADHAN, Nirakar

AU - Dipasquale, Laura

AU - D’Ippolito, Giuliana

AU - Panico, Antonio

AU - Lens, Piet N.L.

AU - Esposito, Giovanni

AU - Fontana, Angelo

PY - 2017/6

Y1 - 2017/6

N2 - Thermotoga neapolitana is a hyperthermophilic eubacterium that produces hydrogen by sugar fermentation. A lab strain of T. neapolitana DSMZ 4359T maintained in a CO2-enriched atmosphere showed a stable increase of lactic acid production under capnophilic lactic fermentation (CLF) conditions. The genotypic comparison between the putative mutant (TN-CMut) and the original strain DSMZ 4359T (WT4359) revealed 88.1 (±2.4)% DNA homology. RiboPrint® and MALDI-TOF mass analyses support a genetic differentiation beyond subspecies level. The phenotypic characterization indicated a high correlation between the two strains, except for the lactic acid production. Under identical operating conditions, the lab mutant produced significantly more lactic acid than the parent strain without impairing the hydrogen yield. The highest divergence between TN-CMut and WT4359 was observed for fermentation of glucose or lactose at 80 °C. Based on these results, we propose that the lab strain is a new subspecies of the genus Thermotoga that is named T. neapolitana subsp. capnolactica with regards to its improved feature to produce lactic acid under capnophilic conditions.

AB - Thermotoga neapolitana is a hyperthermophilic eubacterium that produces hydrogen by sugar fermentation. A lab strain of T. neapolitana DSMZ 4359T maintained in a CO2-enriched atmosphere showed a stable increase of lactic acid production under capnophilic lactic fermentation (CLF) conditions. The genotypic comparison between the putative mutant (TN-CMut) and the original strain DSMZ 4359T (WT4359) revealed 88.1 (±2.4)% DNA homology. RiboPrint® and MALDI-TOF mass analyses support a genetic differentiation beyond subspecies level. The phenotypic characterization indicated a high correlation between the two strains, except for the lactic acid production. Under identical operating conditions, the lab mutant produced significantly more lactic acid than the parent strain without impairing the hydrogen yield. The highest divergence between TN-CMut and WT4359 was observed for fermentation of glucose or lactose at 80 °C. Based on these results, we propose that the lab strain is a new subspecies of the genus Thermotoga that is named T. neapolitana subsp. capnolactica with regards to its improved feature to produce lactic acid under capnophilic conditions.

U2 - 10.1016/j.ijhydene.2017.05.052

DO - 10.1016/j.ijhydene.2017.05.052

M3 - Journal article

SN - 0360-3199

VL - 42

SP - 16023

EP - 16030

JO - International Journal of Hydrogen Energy

JF - International Journal of Hydrogen Energy

IS - 25

ER -

PRADHAN N, Dipasquale L, D’Ippolito G, Panico A, Lens PNL, Esposito G et al. Hydrogen and lactic acid synthesis by the wild-type and a laboratory strain of the hyperthermophilic bacterium Thermotoga neapolitana DSMZ 4359^T under capnophilic lactic fermentation conditions. International Journal of Hydrogen Energy. 2017 Jun;42(25):16023-16030. doi: 10.1016/j.ijhydene.2017.05.052