TY - JOUR
T1 - HPLC Fingerprint analysis and content determination of extract with anticancer activities of sedi linearis herba
AU - Liu, Diangang
AU - Mei, Qing
AU - Long, Weifang
AU - Wan, Xiangluan
AU - Wan, Dingrong
AU - Wang, Luyao
N1 - Funding information:
The author gratefully acknowledges the financial support by the Collaborative Innovation Center of Hubei Province for New Geriatric TCM Products to the Research.
Publisher Copyright:
© 2017 Phcog.Net.
PY - 2017/3
Y1 - 2017/3
N2 - Aims: The purpose of this paper was to develop a HPLC fingerprint analysis method, compare the chromatographic fingerprints characteristics of the ethyl acetate extract with anticancer activity from Sedi Linearis Herba (Sedum lineare Thumb.) collected in different seasons and environments, determine the contents of active ingredients hyperoside, isoquercetin and astragalin of each sample by HPLC, provide believable scientific foundations for quality control and evaluation of Sedi Linearis Herba. Methods: The analyte was analyzed using BDS Hypersil C18 as chromatographic column, acetonitrile -0.1% acetic acid solution as the mobile phase (gradient elution). Results: The results showed that the major active ingredient contents of the samples collected in different seasons but at the same environment all had obvious differences. These ingredient contents of the samples collected in the end of April were much more than those in mid-August in general. Moreover, the major active ingredient contents of the samples picked in the same season but different environments were different too. For instance, the major ingredient contents of the sample in damp environment in August were more than those in sunny environment. Conclusion: All of these indicated that the HPLC fingerprint analysis and contents determination method established were very useful for quality analysis and control of Sedi Linearis Herba.
AB - Aims: The purpose of this paper was to develop a HPLC fingerprint analysis method, compare the chromatographic fingerprints characteristics of the ethyl acetate extract with anticancer activity from Sedi Linearis Herba (Sedum lineare Thumb.) collected in different seasons and environments, determine the contents of active ingredients hyperoside, isoquercetin and astragalin of each sample by HPLC, provide believable scientific foundations for quality control and evaluation of Sedi Linearis Herba. Methods: The analyte was analyzed using BDS Hypersil C18 as chromatographic column, acetonitrile -0.1% acetic acid solution as the mobile phase (gradient elution). Results: The results showed that the major active ingredient contents of the samples collected in different seasons but at the same environment all had obvious differences. These ingredient contents of the samples collected in the end of April were much more than those in mid-August in general. Moreover, the major active ingredient contents of the samples picked in the same season but different environments were different too. For instance, the major ingredient contents of the sample in damp environment in August were more than those in sunny environment. Conclusion: All of these indicated that the HPLC fingerprint analysis and contents determination method established were very useful for quality analysis and control of Sedi Linearis Herba.
KW - Anticancer activity ingredients
KW - Content determination
KW - HPLC fingerprint
KW - Sedum lineare thunb
UR - http://www.scopus.com/inward/record.url?scp=85018509204&partnerID=8YFLogxK
U2 - 10.5530/pj.2017.2.22
DO - 10.5530/pj.2017.2.22
M3 - Journal article
AN - SCOPUS:85018509204
SN - 0975-3575
VL - 9
SP - 128
EP - 134
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 2
ER -