HPLC测定半边莲药材中2个多炔类成分的含量

目的：建立测定半边莲药材中2个多炔类成分lobetyolin和lobetyolinin的HPLC定量分析方法。方法：采用C18柱，乙腈-水（0~25 min,10%~40%乙腈）梯度洗脱，流速1.0 mL.min-1，检测波长267 nm。结果：半边莲药材中2个多炔类成分含量普遍较低，其中最高者含lobetyolin和lobetyolinin分别为0.461,0.436 mg.g-1,总量接近1‰，而在有的样品中未检测到。结论：本方法简便可行，适用于半边莲药材中lobetyolin和lobetyolinin的含量测定。

Objective: To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.

Method: C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.

Result: Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.

Conclusion: A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.