Translated title of the contribution: Determination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis

乔春峰, 贺震旦, 韩全斌, 宋景政, 徐宏喜

Research output: Contribution to journalJournal articlepeer-review

5 Citations (Scopus)


目的:建立测定半边莲药材中2个多炔类成分lobetyolin和lobetyolinin的HPLC定量分析方法。方法:采用C18柱,乙腈-水(0~25 min,10%~40%乙腈)梯度洗脱,流速1.0 mL.min-1,检测波长267 nm。结果:半边莲药材中2个多炔类成分含量普遍较低,其中最高者含lobetyolin和lobetyolinin分别为0.461,0.436 mg.g-1,总量接近1‰,而在有的样品中未检测到。结论:本方法简便可行,适用于半边莲药材中lobetyolin和lobetyolinin的含量测定。

Objective: To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.

Method: C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.

Result: Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.

Conclusion: A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.
Translated title of the contributionDetermination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis
Original languageChinese (Simplified)
Pages (from-to)744-746
Number of pages3
Issue number9
Publication statusPublished - 1 May 2006

User-Defined Keywords

  • 半边莲
  • 多炔
  • 含量测定
  • lobetyolin
  • lobetyolinin
  • Herba Lobeliae Chinensis
  • polyacetylene
  • HPLC
  • determination


Dive into the research topics of 'Determination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis'. Together they form a unique fingerprint.

Cite this