HPLC法同时测定鲜鱼腥草不同部位8个活性成分的含量

目的: 同时测定鲜鱼腥草不同药用部位8个活性成分的含量。

方法: Alltima C18柱(7.5 mm×4.6 mm,5μm),流动相:0.2%的冰乙酸溶液(A),乙腈(B),梯度洗脱:0~20 min,5%~20%B;20~40 min,20%~20.5%B;40~50 min,20.5%~35%B;50~90 min,35%~90%B;流速:0.6 mL/min;检测波长:280 nm。

结果: 表明不同药用部位含量不同。

结论: 该法简便、准确、分离效果好,适用于鱼腥草的质量控制。

Aim: To determine the amounts of the eight bioactive components in the different parts of fresh Houttuynia cordata by HPLC.

Methods: Chromatographic separation of the eight bioactive components was carried out on a Alltima C18 column. The mobile phase consisted of(A) 0.2% acetic acid and(B) acetonitrile using a gradient elution program which was set to 20%(B) in 0～20 min,20%～20.5%(B) in 20～40 min,20.5%～35%(B) in 40～50 min, and 35%～90%(B) in 50～90 min. The flow rate was 0.6 mL/min and the UV detection wavelength was 280 nm.

Results: The amounts of the eight bioactive components in the different parts of H.cordata were found to be varied.

Conclusion: The developed method is simple, accurate, selective, and reliable for the quality control of H.cordata.