Highly sensitive immunoassays through dielectrophoresisbased protein enrichment using integrated nanorods

Zhen Cao*, Jiongdong Zhao, Yang Liu, Junxue Fu

*Corresponding author for this work

Research output: Chapter in book/report/conference proceedingConference proceedingpeer-review

Abstract

Here, we present a novel insulator-based dielectrophoresis (DEP) device integrated with nanorod arrays for highly effective and rapid protein enrichment and detection. Dense arrays of Ag nanorods coated with oxide are fabricated on glass wafers prepatterned with microelectrodes; therefore creates a super high electric field gradient and concomitant adequate dielectrophoresis force for trapping of proteins. The fabrication process exploits oblique angle deposition (OAD), a physical vapor deposition method, for easy integration of nanostructures in DEP devices. This technique employs the shadowing effect and surface diffusion of adatoms, which eliminates the requirement of advanced lithography equipment. Leveraging on this platform, enrichment of bovine serum albumin (BSA) protein is demonstrated by 1800-fold with 180-fold/s when only applying 5 V electric potential at 1MHz. Further, the immunoassay of a tumor biomarker, carcinoembryonic antigen (CEA), with femtomolar sensitivity (~7.5 fM) is realized using this platform, referring to a 4,300-fold enhancement in the detection limit compared with identical assays performed on blank glass plates. The enrichment of proteins also accelerates the binding kinetics and enables signal saturation within one minute.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages358-361
Number of pages4
ISBN (Electronic)9781510897571
Publication statusPublished - 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: 11 Nov 201815 Nov 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume1

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan, Province of China
CityKaohsiung
Period11/11/1815/11/18

Scopus Subject Areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

User-Defined Keywords

  • Dielectrophoresis
  • Immunoassay
  • Oblique angle deposition
  • Protein enrichment

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