Highly Sensitive Graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H2O2 Detection

Yuanyuan Zhang, Xiaoyun Bai, Xuemei Wang*, Kwok Keung SHIU, Yanliang Zhu, Hui Jiang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

202 Citations (Scopus)

Abstract

A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M-1 cm-2, n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.

Original languageEnglish
Pages (from-to)9459-9465
Number of pages7
JournalAnalytical Chemistry
Volume86
Issue number19
DOIs
Publication statusPublished - 7 Oct 2014

Scopus Subject Areas

  • Analytical Chemistry

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