Highly Sensitive Graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H2O2 Detection

Yuanyuan Zhang; Xiaoyun Bai; Xuemei Wang; Kwok Keung Shiu; Yanliang Zhu; Hui Jiang

doi:10.1021/ac5009699

Highly Sensitive Graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H₂O₂ Detection

Yuanyuan Zhang, Xiaoyun Bai, Xuemei Wang^*, Kwok Keung Shiu^*, Yanliang Zhu, Hui Jiang

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

280 Citations (Scopus)

Abstract

A sensitive hydrogen peroxide (H₂O₂) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H₂O₂ from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K₂PtCl₆ solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H₂O₂. The relevant detection limit of H₂O₂ is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M^-1 cm^-2, n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H₂O₂ release from living cells because of its low detection limit, wide linear range, and higher sensitivity.

Original language	English
Pages (from-to)	9459-9465
Number of pages	7
Journal	Analytical Chemistry
Volume	86
Issue number	19
DOIs	https://doi.org/10.1021/ac5009699
Publication status	Published - 7 Oct 2014

Scopus Subject Areas

Analytical Chemistry

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10.1021/ac5009699

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@article{c8ec0c74db2c4f8fb7a1a8be05f84937,

title = "Highly Sensitive Graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H2O2 Detection",

abstract = "A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M-1 cm-2, n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.",

author = "Yuanyuan Zhang and Xiaoyun Bai and Xuemei Wang and Shiu, {Kwok Keung} and Yanliang Zhu and Hui Jiang",

note = "Funding information: This work is supported by the National Basic Research Program (2010CB732404), National Science Foundation of China (81325011, 21175020), the National High Technology Research and Development Program of China (2012AA022703), Suzhou Science & Technology Major Project (ZXY2012028), and the Faculty Research Grants of HKBU (FRG2/11-12/078). Publisher copyright: {\textcopyright} 2014 American Chemical Society",

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T1 - Highly Sensitive Graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H2O2 Detection

AU - Zhang, Yuanyuan

AU - Bai, Xiaoyun

AU - Wang, Xuemei

AU - Shiu, Kwok Keung

AU - Zhu, Yanliang

AU - Jiang, Hui

N1 - Funding information: This work is supported by the National Basic Research Program (2010CB732404), National Science Foundation of China (81325011, 21175020), the National High Technology Research and Development Program of China (2012AA022703), Suzhou Science & Technology Major Project (ZXY2012028), and the Faculty Research Grants of HKBU (FRG2/11-12/078). Publisher copyright: © 2014 American Chemical Society

PY - 2014/10/7

Y1 - 2014/10/7

N2 - A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M-1 cm-2, n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.

AB - A sensitive hydrogen peroxide (H2O2) sensor was constructed based on graphene-Pt (RGO-Pt) nanocomposites and used to measure the release of H2O2 from living cells. The graphene and Pt nanoparticles (Pt NPs) were modified on glassy carbon electrode (GCE) by the physical adsorption and electrodeposition of K2PtCl6 solution, respectively. Through characterization by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), it was observed that the electrodeposited Pt NPs were densely covered and well distributed on the entire graphene surface. Electrochemical study demonstrates that the RGO-Pt nanocomposites modified glassy carbon electrode exhibited a high peak current and low overpotential toward the reduction of H2O2. The relevant detection limit of H2O2 is ∼0.2 μM with a wide linear range from 0.5 μM to 3.475 mM, displaying a much higher sensitivity (459 ± 3 mA M-1 cm-2, n = 5) than that of Pt nanoparticles or graphene modified electrode. This novel biosensor can measure the H2O2 release from living cells because of its low detection limit, wide linear range, and higher sensitivity.

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Highly Sensitive Graphene-Pt Nanocomposites Amperometric Biosensor and Its Application in Living Cell H₂O₂ Detection

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