TY - JOUR
T1 - Highly sensitive fluorescent immunosensor for detection of influenza virus based on Ag autocatalysis
AU - Li, Yanxia
AU - Hong, Mei
AU - Qiu, Bin
AU - Lin, Zhenyu
AU - Chen, Yiting
AU - CAI, Zongwei
AU - Chen, Guonan
N1 - Funding Information:
This project was financially supported by the National Basic Research Program of China (No. 2010CB732403 ), NSFC ( 21275031 , 41076059 , 20928005 , 20905034 and 21175025 ), the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1116 ), and the Fujian Province Natural Science Foundation ( 2013J01052 ), and the Science and Technology project of education department of Fujian province ( JA11194 ).
PY - 2014/4/15
Y1 - 2014/4/15
N2 - A versatile, ultrasensitive immunosensor for detection of influenza virus was designed by combining silver nanoparticles (Ag NPs) labeled antibodies with indirect fluorescence. A new technology using Ag-S covalent binding was applied for antibody labeling. Influenza A (H1N1) virus, as a subtype of influenza A virus that was the most common cause of human influenza (flu), was acted as the target antigen using sandwich type-immunoreactions on the high binding ELISA plates. The antibody-labeled Ag NPs were then released by acid solution to produce Ag+ which can catalyze o-phenylenediamine (OPDA) oxidation to produce fluorescence for highly sensitive detection. Under the optimal conditions, it shows good linear relationship between fluorescence intensity and the logarithm of the concentration of H1N1 over the range of 1.0×10-12-1.0×10-8gmL-1 with a detection limit (LOD, 3σ) of 1.0×10-13gmL-1. Results indicated that the proposed method give a good sensitivity and simple operation for detecting the influenza virus. This work also provided a promising potential for antigen detection by Ag NPs labeled, and the steps were easy to handle.
AB - A versatile, ultrasensitive immunosensor for detection of influenza virus was designed by combining silver nanoparticles (Ag NPs) labeled antibodies with indirect fluorescence. A new technology using Ag-S covalent binding was applied for antibody labeling. Influenza A (H1N1) virus, as a subtype of influenza A virus that was the most common cause of human influenza (flu), was acted as the target antigen using sandwich type-immunoreactions on the high binding ELISA plates. The antibody-labeled Ag NPs were then released by acid solution to produce Ag+ which can catalyze o-phenylenediamine (OPDA) oxidation to produce fluorescence for highly sensitive detection. Under the optimal conditions, it shows good linear relationship between fluorescence intensity and the logarithm of the concentration of H1N1 over the range of 1.0×10-12-1.0×10-8gmL-1 with a detection limit (LOD, 3σ) of 1.0×10-13gmL-1. Results indicated that the proposed method give a good sensitivity and simple operation for detecting the influenza virus. This work also provided a promising potential for antigen detection by Ag NPs labeled, and the steps were easy to handle.
KW - Fluorescent immunoassay
KW - H1N1 Influenza virus
KW - OPDA
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84888415445&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2013.10.045
DO - 10.1016/j.bios.2013.10.045
M3 - Journal article
C2 - 24292140
AN - SCOPUS:84888415445
SN - 0956-5663
VL - 54
SP - 358
EP - 364
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -
