Highly sensitive amperometric glucose biosensor based on glassy carbon electrode with copper/palladium coating

Yi Qing Dai, Kwok Keung SHIU*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

27 Citations (Scopus)


A highly sensitive amperometric glucose biosensor based on immobilizing glucose oxidase in electropolymerized poly(o-phenylenediamine) film on glassy carbon electrode coated sequentially with copper and palladium layers has been developed. The steady-state amperometric response to glucose was determined by means of the oxidation of hydrogen peroxide generated by the enzymatic reaction at a potential of either +0.70 or +0.40 V (vs. Ag | AgCl reference). The deposited copper/palladium layer showed great enhancement in the performance of the enzyme electrode, possibly due to its better electrocatalytic activity for hydrogen peroxide oxidation and large surface area. Effects of the relative loading of palladium, enzyme and polymer on the electrode performance were examined in detail. Sensitivity and detection limit for glucose determinations at +0.70 V were about 7.3 μA/mM and 0.1 μM, respectively. A wide linear range up to 6.0 mM glucose could be achieved. Electrode performance was superior to similar works reported in the literature. The response time was less than 2 s and its lifetime was longer than three months. The permeable polyphenylenediamine film also offered good anti-interference ability to ascorbic acid, uric acid and acetaminophen, especially when a detection potential of +0.40 V was employed.

Original languageEnglish
Pages (from-to)1806-1813
Number of pages8
Issue number21
Publication statusPublished - Nov 2004

Scopus Subject Areas

  • Analytical Chemistry
  • Electrochemistry

User-Defined Keywords

  • Amperometric biosensor
  • Copper undercoating
  • Glucose determination
  • Palladium deposition
  • Poly(o-phenylenediamine) film


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