TY - JOUR
T1 - Highly Selective Two-Photon Fluorescent Probe for Ratiometric Sensing and Imaging Cysteine in Mitochondria
AU - Niu, Weifen
AU - Guo, Lei
AU - Li, Yinhui
AU - Shuang, Shaomin
AU - Dong, Chuan
AU - Wong, Man Shing
N1 - Funding information:
This work was supported by the National Natural Science Foundation of China (Nos. 21475080, 21575084, and 21305036), the Hundred Talent Program of Shanxi Province, Shanxi Province Scholarship Council of China and Natural Science Foundation of Chongqing Municipal Education Commission (No. KJ1401210). This work was also financially supported by the General Research Fund (HKBU 203212), Hong Kong Research Grant Council, FRG (FRG2/13-14/059), 2014 Hong Kong Scholars Program, and Institute of Molecular Functional Materials, which was supported by a grant from the University Grants Committee, Areas of Excellence Scheme (AoE/P-03/08).
Publisher copyright:
© 2015 American Chemical Society
PY - 2016/2/2
Y1 - 2016/2/2
N2 - A novel ratiometric mitochondrial cysteine (Cys)-selective two-photon fluorescence probe has been developed on the basis of a merocyanine as the fluorophore and an acrylate moiety as the biothiol reaction site. The biocompatible and photostable acrylate-functionalized merocyanine probe shows not only a mitochondria-targeting property but also highly selective detection and monitoring of Cys over other biothiols such as homocysteine (Hcy) and glutathione (GSH) and hydrogen sulfide (H2S) in live cells. In addition, this probe exhibits ratiometric fluorescence emission characteristics (F518/F452), which are linearly proportional to Cys concentrations in the range of 0.5-40μM. More importantly, the probe and its released fluorophore, merocyanine, exhibit strong two-photon excited fluorescence (TPEF) with two-photon action cross-section (σmax) of 65.2 GM at 740 nm and 72.6 GM at 760 nm in aqueous medium, respectively, which is highly desirable for high contrast and brightness ratiometric two-photon fluorescence imaging of the living samples. The probe has been successfully applied to ratiometrically image and detect mitochondrial Cys in live cells and intact tissues down to a depth of 150μm by two-photon fluorescence microscopy. Thus, this ratiometric two-photon fluorescent probe is practically useful for an investigation of Cys in living biological systems.
AB - A novel ratiometric mitochondrial cysteine (Cys)-selective two-photon fluorescence probe has been developed on the basis of a merocyanine as the fluorophore and an acrylate moiety as the biothiol reaction site. The biocompatible and photostable acrylate-functionalized merocyanine probe shows not only a mitochondria-targeting property but also highly selective detection and monitoring of Cys over other biothiols such as homocysteine (Hcy) and glutathione (GSH) and hydrogen sulfide (H2S) in live cells. In addition, this probe exhibits ratiometric fluorescence emission characteristics (F518/F452), which are linearly proportional to Cys concentrations in the range of 0.5-40μM. More importantly, the probe and its released fluorophore, merocyanine, exhibit strong two-photon excited fluorescence (TPEF) with two-photon action cross-section (σmax) of 65.2 GM at 740 nm and 72.6 GM at 760 nm in aqueous medium, respectively, which is highly desirable for high contrast and brightness ratiometric two-photon fluorescence imaging of the living samples. The probe has been successfully applied to ratiometrically image and detect mitochondrial Cys in live cells and intact tissues down to a depth of 150μm by two-photon fluorescence microscopy. Thus, this ratiometric two-photon fluorescent probe is practically useful for an investigation of Cys in living biological systems.
UR - http://www.scopus.com/inward/record.url?scp=84957555978&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.5b04329
DO - 10.1021/acs.analchem.5b04329
M3 - Article
C2 - 26717855
AN - SCOPUS:84957555978
SN - 0003-2700
VL - 88
SP - 1908
EP - 1914
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 3
ER -