Highly efficient transgenesis with miniMos in Caenorhabditis briggsae

Qiutao Ding, Xiaoliang Ren, Runsheng LI, Luyan Chan, Vincy Wing Sze HO, Yu BI, Dongying Xie, Zhongying Zhao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Caenorhabditis briggsae as a companion species for Caenorhabditis elegans has played an increasingly important role in study of evolution of development and genome and gene regulation. Aided by the isolation of its sister spices, it has recently been established as a model for speciation study. To take full advantage of the species for comparative study, an effective transgenesis method especially those with single-copy insertion is important for functional comparison. Here, we improved a transposon-based transgenesis methodology that had been originally developed in C. elegans but worked marginally in C. briggsae. By incorporation of a heat shock step, the transgenesis efficiency in C. briggsae with a single-copy insertion is comparable to that in C. elegans. We used the method to generate 54 independent insertions mostly consisting of a mCherry tag over the C. briggsae genome. We demonstrated the use of the tags in identifying interacting loci responsible for hybrid male sterility between C. briggsae and Caenorhabditis nigoni when combined with the GFP tags we generated previously. Finally, we demonstrated that C. briggsae tolerates the C. elegans toxin, PEEL-1, but not SUP-35, making the latter a potential negative selection marker against extrachromosomal array.

Original languageEnglish
Article numberjkac254
JournalG3: Genes, Genomes, Genetics
Volume12
Issue number12
DOIs
Publication statusPublished - Dec 2022

Scopus Subject Areas

  • Genetics(clinical)
  • Genetics
  • Molecular Biology

User-Defined Keywords

  • Caenorhabditis briggsae
  • heat shock
  • miniMos
  • transgenesis

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