High Throughput Screening of Signal Transduction Mutants with Luciferase Imaging

Liming Xiong; Lisa David; Becky Stevenson; Jian Kang Zhu

doi:10.1023/A:1007519200505

High Throughput Screening of Signal Transduction Mutants with Luciferase Imaging

Liming Xiong, Lisa David, Becky Stevenson, Jian Kang Zhu^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

20 Citations (Scopus)

Abstract

A detailed procedure for high throughput genetic screening of hormone and environmental stress signal transduction mutants of Arabidopsis thaliana is described. The screen was carried out with mutagenized plants expressing the firefly luciferase reporter under control of a cold, osmotic stress, and absciscic acid responsive promoter. A thermoelectrically cooled CCD camera was used to detect luminescence emitted by the plants in response to stresses or ABA. Advantages of the screening procedure include high throughput, capability to identify low as well as high expression mutants and employment of a highly sensitive but affordable imaging system and software. This procedure can be used to study complex signal transduction networks in higher plants.

Original language	English
Pages (from-to)	159-170
Number of pages	12
Journal	Plant Molecular Biology Reporter
Volume	17
Issue number	2
DOIs	https://doi.org/10.1023/A:1007519200505
Publication status	Published - Jun 1999

Scopus Subject Areas

Molecular Biology
Plant Science

User-Defined Keywords

CCD camera
Imaging
Luciferase
Mutant screening
Signal transduction

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10.1023/A:1007519200505

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title = "High Throughput Screening of Signal Transduction Mutants with Luciferase Imaging",

abstract = "A detailed procedure for high throughput genetic screening of hormone and environmental stress signal transduction mutants of Arabidopsis thaliana is described. The screen was carried out with mutagenized plants expressing the firefly luciferase reporter under control of a cold, osmotic stress, and absciscic acid responsive promoter. A thermoelectrically cooled CCD camera was used to detect luminescence emitted by the plants in response to stresses or ABA. Advantages of the screening procedure include high throughput, capability to identify low as well as high expression mutants and employment of a highly sensitive but affordable imaging system and software. This procedure can be used to study complex signal transduction networks in higher plants.",

keywords = "CCD camera, Imaging, Luciferase, Mutant screening, Signal transduction",

author = "Liming Xiong and Lisa David and Becky Stevenson and Zhu, {Jian Kang}",

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AU - Xiong, Liming

AU - David, Lisa

AU - Stevenson, Becky

AU - Zhu, Jian Kang

PY - 1999/6

Y1 - 1999/6

N2 - A detailed procedure for high throughput genetic screening of hormone and environmental stress signal transduction mutants of Arabidopsis thaliana is described. The screen was carried out with mutagenized plants expressing the firefly luciferase reporter under control of a cold, osmotic stress, and absciscic acid responsive promoter. A thermoelectrically cooled CCD camera was used to detect luminescence emitted by the plants in response to stresses or ABA. Advantages of the screening procedure include high throughput, capability to identify low as well as high expression mutants and employment of a highly sensitive but affordable imaging system and software. This procedure can be used to study complex signal transduction networks in higher plants.

AB - A detailed procedure for high throughput genetic screening of hormone and environmental stress signal transduction mutants of Arabidopsis thaliana is described. The screen was carried out with mutagenized plants expressing the firefly luciferase reporter under control of a cold, osmotic stress, and absciscic acid responsive promoter. A thermoelectrically cooled CCD camera was used to detect luminescence emitted by the plants in response to stresses or ABA. Advantages of the screening procedure include high throughput, capability to identify low as well as high expression mutants and employment of a highly sensitive but affordable imaging system and software. This procedure can be used to study complex signal transduction networks in higher plants.

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KW - Imaging

KW - Luciferase

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KW - Signal transduction

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JO - Plant Molecular Biology Reporter

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High Throughput Screening of Signal Transduction Mutants with Luciferase Imaging

Abstract

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