@article{0d8d71977f7b4cf3a8ed2155898bcaf2,
title = "High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans",
abstract = "Using DNA sequences 5′ to open reading frames, we have constructed green fluorescent protein (GFP) fusions and generated spatial and temporal tissue expression profiles for 1,886 specific genes in the nematode Caenorhabditis elegans. This effort encompasses about 10% of all genes identified in this organism. GFP-expressing wild-type animals were analyzed at each stage of development from embryo to adult. We have identified 5′ DNA regions regulating expression at all developmental stages and in 38 different cell and tissue types in this organism. Among the regulatory regions identified are sequences that regulate expression in all cells, in specific tissues, in combinations of tissues, and in single cells. Most of the genes we have examined in C. elegans have human orthologs. All the images and expression pattern data generated by this project are available at WormAtlas (http://gfpweb.aecom.yu.edu/index) and through WormBase (http://www.wormbase.org).",
author = "Rebecca Hunt-Newbury and Ryan Viveiros and Robert Johnsen and Allan Mah and Dina Anastas and Lily Fang and Erin Halfnight and David Lee and John Lin and Adam Lorch and Sheldon McKay and Okada, {H. Mark} and Jie Pan and Schulz, {Ana K} and Domena Tu and Kim Wong and Z Zhao and Andrey Alexeyenko and Thomas Burglin and Eric Sonnhammer and Ralf Schnabel and Jones, {Steven J} and Marra, {Marco A} and Baillie, {David L} and Moerman, {Donald G}",
note = "Funding information: This research was supported by a grant from Genome Canada and Genome British Columbia to DLB, SJJ, MAM, and DGM. We thank the several members of the C. elegans community who have given of their time to help analyze the expression patterns of the various strains reported here. We especially wish to acknowledge the extensive help and enthusiasm we obtained from David Hall and Zeynep Altun of WormAtlas. We thank Oliver Hobert, Denis Dupuy, Harald Hutter, and Jim McGhee for comments on the manuscript, and Anca Petrescu for bioinformatic advice. We also thank three anonymous reviewers for their thoughtful comments that helped to improve the manuscript. DLB is supported by a Canada Research Chair, and AM is supported by a National Science and Engineering Research Council (NSERC) scholarship. SJJ and MAM are scholars of Michael Smith Foundation for Health Research. In addition, NSERC is supporting genetic strain maintenance. Publisher copyright: {\textcopyright} 2007 Hunt-Newbury et al.",
year = "2007",
month = sep,
doi = "10.1371/journal.pbio.0050237",
language = "English",
volume = "5",
journal = "PLoS Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "9",
}