High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source

Bo Yao, Huihua Yang, Qionglin Liang, Guoan Luo*, Liduo Wang, Kangning REN, Yudi Gao, Yiming Wang, Yong Qiu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

An integrated and simplified microfluidic device using a 250 μm × 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric focusing (IEF) of R-phycoerythrin in a microchip. The IEF conditions were optimized, and the total analysis time was extremely reduced to 30 s for 2-cm-long microchannels at 700 V/cm of electric field strength without the presence of electroosmotic flow. The compression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when 1% hydroxylpropylmethyl cellulose in 2% ampholyte was used as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was ∼0.6 μg/mL or 45 pg at 75 nL/column injection of R-phycoerythrin. This OLED-induced fluorescence detection system for WCID provides a high-speed IEF technique with quantitative ability and the potential for high integration and throughput microchip systems.

Original languageEnglish
Pages (from-to)5845-5850
Number of pages6
JournalAnalytical Chemistry
Volume78
Issue number16
DOIs
Publication statusPublished - 15 Aug 2006

Scopus Subject Areas

  • Analytical Chemistry

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