High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source

Bo Yao; Huihua Yang; Qionglin Liang; Guoan Luo; Liduo Wang; Kangning REN; Yudi Gao; Yiming Wang; Yong Qiu

doi:10.1021/ac060445r

High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source

Bo Yao, Huihua Yang, Qionglin Liang, Guoan Luo^*, Liduo Wang, Kangning REN, Yudi Gao, Yiming Wang, Yong Qiu

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

55 Citations (Scopus)

Abstract

An integrated and simplified microfluidic device using a 250 μm × 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric focusing (IEF) of R-phycoerythrin in a microchip. The IEF conditions were optimized, and the total analysis time was extremely reduced to 30 s for 2-cm-long microchannels at 700 V/cm of electric field strength without the presence of electroosmotic flow. The compression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when 1% hydroxylpropylmethyl cellulose in 2% ampholyte was used as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was ∼0.6 μg/mL or 45 pg at 75 nL/column injection of R-phycoerythrin. This OLED-induced fluorescence detection system for WCID provides a high-speed IEF technique with quantitative ability and the potential for high integration and throughput microchip systems.

Original language	English
Pages (from-to)	5845-5850
Number of pages	6
Journal	Analytical Chemistry
Volume	78
Issue number	16
DOIs	https://doi.org/10.1021/ac060445r
Publication status	Published - 15 Aug 2006

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Analytical Chemistry

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@article{327103f3063e47668aa1a1b8efe5562c,

title = "High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source",

abstract = "An integrated and simplified microfluidic device using a 250 μm × 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric focusing (IEF) of R-phycoerythrin in a microchip. The IEF conditions were optimized, and the total analysis time was extremely reduced to 30 s for 2-cm-long microchannels at 700 V/cm of electric field strength without the presence of electroosmotic flow. The compression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when 1% hydroxylpropylmethyl cellulose in 2% ampholyte was used as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was ∼0.6 μg/mL or 45 pg at 75 nL/column injection of R-phycoerythrin. This OLED-induced fluorescence detection system for WCID provides a high-speed IEF technique with quantitative ability and the potential for high integration and throughput microchip systems.",

author = "Bo Yao and Huihua Yang and Qionglin Liang and Guoan Luo and Liduo Wang and Kangning REN and Yudi Gao and Yiming Wang and Yong Qiu",

note = "This research was supported by projects of the National Science Foundations of China (Grant 20299036, 20475031), National Basic Research Program (973 Program) of China (2005CB523503, 2001CB510305), and Key Technology Program of China (2004BA721A13). ",

year = "2006",

month = aug,

day = "15",

doi = "10.1021/ac060445r",

language = "English",

volume = "78",

pages = "5845--5850",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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TY - JOUR

T1 - High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source

AU - Yao, Bo

AU - Yang, Huihua

AU - Liang, Qionglin

AU - Luo, Guoan

AU - Wang, Liduo

AU - REN, Kangning

AU - Gao, Yudi

AU - Wang, Yiming

AU - Qiu, Yong

N1 - This research was supported by projects of the National Science Foundations of China (Grant 20299036, 20475031), National Basic Research Program (973 Program) of China (2005CB523503, 2001CB510305), and Key Technology Program of China (2004BA721A13).

PY - 2006/8/15

Y1 - 2006/8/15

N2 - An integrated and simplified microfluidic device using a 250 μm × 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric focusing (IEF) of R-phycoerythrin in a microchip. The IEF conditions were optimized, and the total analysis time was extremely reduced to 30 s for 2-cm-long microchannels at 700 V/cm of electric field strength without the presence of electroosmotic flow. The compression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when 1% hydroxylpropylmethyl cellulose in 2% ampholyte was used as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was ∼0.6 μg/mL or 45 pg at 75 nL/column injection of R-phycoerythrin. This OLED-induced fluorescence detection system for WCID provides a high-speed IEF technique with quantitative ability and the potential for high integration and throughput microchip systems.

AB - An integrated and simplified microfluidic device using a 250 μm × 1-4 cm of organic light emitting diode (OLED) array as a two-dimensional light source for single-channel and multichannel whole-column imaging detection was developed. This fluorescence detection system was used for isoelectric focusing (IEF) of R-phycoerythrin in a microchip. The IEF conditions were optimized, and the total analysis time was extremely reduced to 30 s for 2-cm-long microchannels at 700 V/cm of electric field strength without the presence of electroosmotic flow. The compression of pH gradient caused by electrolytes drawing into the microchannels was efficiently restrained when 1% hydroxylpropylmethyl cellulose in 2% ampholyte was used as the carrier for IEF. Under optimized IEF conditions, the detection limit of this system was ∼0.6 μg/mL or 45 pg at 75 nL/column injection of R-phycoerythrin. This OLED-induced fluorescence detection system for WCID provides a high-speed IEF technique with quantitative ability and the potential for high integration and throughput microchip systems.

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U2 - 10.1021/ac060445r

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JO - Analytical Chemistry

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High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source

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