High-performance liquid chromatography coupled with tandem mass spectrometry applied for metabolic study of ginsenoside Rb1 on rat

Tianxiu Qian; Zhi Hong JIANG; Zongwei CAI

doi:10.1016/j.ab.2006.02.025

High-performance liquid chromatography coupled with tandem mass spectrometry applied for metabolic study of ginsenoside Rb₁ on rat

Tianxiu Qian, Zhi Hong JIANG, Zongwei CAI^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

72 Citations (Scopus)

Abstract

Liquid chromatography coupled with mass spectrometry and tandem mass spectrometry has been applied to investigate the in vivo metabolism of ginsenoside Rb₁ in rat. Both positive electrospray ionization mass spectrometry and negative electrospray ionization mass spectrometry were used to identify the Rb₁ and its metabolites in rat plasma, urine, and feces samples. Oxygenation and deglycosylation were found to be the major metabolic pathways of Rb₁ in rat. A total of nine metabolites were detected in urine and feces samples collected after intravenous and oral administration. Deglycosylated metabolism of Rb₁ generated other ginsenosides as the major metabolites, such as Rd, Rg₃ or F₂, Rh₂, or C-K. This result indicates that the ginsenoside Rb₁ has many pharmacological activities and could be used as a prodrug.

Original language	English
Pages (from-to)	87-96
Number of pages	10
Journal	Analytical Biochemistry
Volume	352
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2006.02.025
Publication status	Published - 1 May 2006

Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

User-Defined Keywords

Ginsenoside Rb
In vivo metabolites
LC-MS-MS
Rat

Access to Document

10.1016/j.ab.2006.02.025

Cite this

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title = "High-performance liquid chromatography coupled with tandem mass spectrometry applied for metabolic study of ginsenoside Rb1 on rat",

abstract = "Liquid chromatography coupled with mass spectrometry and tandem mass spectrometry has been applied to investigate the in vivo metabolism of ginsenoside Rb1 in rat. Both positive electrospray ionization mass spectrometry and negative electrospray ionization mass spectrometry were used to identify the Rb1 and its metabolites in rat plasma, urine, and feces samples. Oxygenation and deglycosylation were found to be the major metabolic pathways of Rb1 in rat. A total of nine metabolites were detected in urine and feces samples collected after intravenous and oral administration. Deglycosylated metabolism of Rb1 generated other ginsenosides as the major metabolites, such as Rd, Rg3 or F2, Rh2, or C-K. This result indicates that the ginsenoside Rb1 has many pharmacological activities and could be used as a prodrug.",

keywords = "Ginsenoside Rb, In vivo metabolites, LC-MS-MS, Rat",

author = "Tianxiu Qian and JIANG, {Zhi Hong} and Zongwei CAI",

note = "Funding Information: This work was supported by a Faculty Research Grant provided by HKBU and earmarked Grants (HKBU HKBU2017/02P and HKBU2154/04M) obtained from RGC, University Grants Committee of Hong Kong.",

year = "2006",

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AU - Qian, Tianxiu

AU - JIANG, Zhi Hong

AU - CAI, Zongwei

N1 - Funding Information: This work was supported by a Faculty Research Grant provided by HKBU and earmarked Grants (HKBU HKBU2017/02P and HKBU2154/04M) obtained from RGC, University Grants Committee of Hong Kong.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - Liquid chromatography coupled with mass spectrometry and tandem mass spectrometry has been applied to investigate the in vivo metabolism of ginsenoside Rb1 in rat. Both positive electrospray ionization mass spectrometry and negative electrospray ionization mass spectrometry were used to identify the Rb1 and its metabolites in rat plasma, urine, and feces samples. Oxygenation and deglycosylation were found to be the major metabolic pathways of Rb1 in rat. A total of nine metabolites were detected in urine and feces samples collected after intravenous and oral administration. Deglycosylated metabolism of Rb1 generated other ginsenosides as the major metabolites, such as Rd, Rg3 or F2, Rh2, or C-K. This result indicates that the ginsenoside Rb1 has many pharmacological activities and could be used as a prodrug.

AB - Liquid chromatography coupled with mass spectrometry and tandem mass spectrometry has been applied to investigate the in vivo metabolism of ginsenoside Rb1 in rat. Both positive electrospray ionization mass spectrometry and negative electrospray ionization mass spectrometry were used to identify the Rb1 and its metabolites in rat plasma, urine, and feces samples. Oxygenation and deglycosylation were found to be the major metabolic pathways of Rb1 in rat. A total of nine metabolites were detected in urine and feces samples collected after intravenous and oral administration. Deglycosylated metabolism of Rb1 generated other ginsenosides as the major metabolites, such as Rd, Rg3 or F2, Rh2, or C-K. This result indicates that the ginsenoside Rb1 has many pharmacological activities and could be used as a prodrug.

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