High-performance liquid chromatography coupled with mass spectrometry for analysis of ultrasmall palladium nanoparticles

Lei Zhang, Zhongping Li, Yan Zhang, Man Chin Paau, Qin Hu, Xiaojuan Gong, Shaomin Shuang, Chuan Dong*, Xiaoguang Peng, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

10 Citations (Scopus)


Metal nanoparticles (NPs) have recently attracted considerable attention in many areas of research including bioscience, chemistry and material science. Regrettably, most current and past work usually focuses on studies of multi-component NPs mixture where there is a plethora of NPs species co-existing. This work highlights the merits of reverse-phase high-performance liquid chromatography (RP-HPLC) for disclosing the genuine properties of individual palladium nanoparticles (PdNPs) species present in an as-synthesized N,N′-dimethylformamide-stabilized PdNPs product (DMF-PdNPs) which might have been previously hidden or misinterpreted. DMF-PdNPs is successfully separated by RP-HPLC that smaller DMF-PdNPs are approximately eluted first and then follow by the large ones on a C18 column. The separation fractions are further collected and determined their chemical compositions by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results unambiguously reveal that the as-synthesized DMF-PdNPs product is indeed a complex mixture of ultrasmall PdxNPs (x=10-20) stabilized with different numbers of DMF ligands. It is anticipated that the separated fractions afforded by RP-HPLC will offer more accurate determinations of the catalytic, electronic, optical and toxicological properties of metal NPs which might have been previously misinterpreted.

Original languageEnglish
Pages (from-to)632-639
Number of pages8
Publication statusPublished - Jan 2015

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Analytical separation
  • Chemical composition
  • Metal nanoparticles


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