High-performance liquid chromatographic analysis of as-synthesised N,N′-dimethylformamide-stabilised gold nanoclusters product

Shunping Xie; Man Chin Paau; Yan Zhang; Shaomin Shuang; Wan Chan; Martin M.F. Choi

doi:10.1039/c2nr30984j

High-performance liquid chromatographic analysis of as-synthesised N,N′-dimethylformamide-stabilised gold nanoclusters product

Shunping Xie
, Man Chin Paau
, Yan Zhang
, Shaomin Shuang^*
, Wan Chan
, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

34 Citations (Scopus)

Abstract

Reverse-phase high-performance liquid chromatographic (RP-HPLC) separation and analysis of polydisperse water-soluble gold nanoclusters (AuNCs) stabilised with N,N′-dimethylformamide (DMF) were investigated. Under optimal elution gradient conditions, the separation of DMF-AuNCs was monitored by absorption and fluorescence spectroscopy. The UV-vis spectral characteristics of the separated DMF-AuNCs have been captured and they do not possess distinct surface plasmon resonance bands, indicating that all DMF-AuNCs are small AuNCs. The photoluminescence emission spectra of the separated DMF-AuNCs are in the blue-light region. Moreover, cationic DMF-AuNCs are for the first time identified by ion chromatography. Our proposed RP-HPLC methodology has been successfully applied to separate AuNCs of various Au atoms as well as DMF-stabilised ligands. Finally, the composition of the separated DMF-AuNCs was confirmed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and electrospray ionisation mass spectrometry, proving that the as-synthesised DMF-AuNCs product consists of Au₁₀⁺, Au₁₀, Au₁₁, Au₁₂, Au₁₃, and Au ₁₄ NCs stabilised with various numbers of DMF ligands.

Original language	English
Pages (from-to)	5325-5332
Number of pages	8
Journal	Nanoscale
Volume	4
Issue number	17
DOIs	https://doi.org/10.1039/c2nr30984j
Publication status	Published - 7 Sept 2012

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@article{c178c912bedc49d48fdf9befa7622408,

title = "High-performance liquid chromatographic analysis of as-synthesised N,N′-dimethylformamide-stabilised gold nanoclusters product",

abstract = "Reverse-phase high-performance liquid chromatographic (RP-HPLC) separation and analysis of polydisperse water-soluble gold nanoclusters (AuNCs) stabilised with N,N′-dimethylformamide (DMF) were investigated. Under optimal elution gradient conditions, the separation of DMF-AuNCs was monitored by absorption and fluorescence spectroscopy. The UV-vis spectral characteristics of the separated DMF-AuNCs have been captured and they do not possess distinct surface plasmon resonance bands, indicating that all DMF-AuNCs are small AuNCs. The photoluminescence emission spectra of the separated DMF-AuNCs are in the blue-light region. Moreover, cationic DMF-AuNCs are for the first time identified by ion chromatography. Our proposed RP-HPLC methodology has been successfully applied to separate AuNCs of various Au atoms as well as DMF-stabilised ligands. Finally, the composition of the separated DMF-AuNCs was confirmed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and electrospray ionisation mass spectrometry, proving that the as-synthesised DMF-AuNCs product consists of Au10+, Au10, Au11, Au12, Au13, and Au 14 NCs stabilised with various numbers of DMF ligands.",

author = "Shunping Xie and Paau, \{Man Chin\} and Yan Zhang and Shaomin Shuang and Wan Chan and Choi, \{Martin M.F.\}",

note = "Financial supports from the Hundred Talent Programme of Shanxi Province and HKBU Faculty Research Grant (FRG2/10-11/112) are gratefully acknowledged. M. C. Paau acknowledges the receipt of a postgraduate studentship from the Additional UGC-funded Research Postgraduate Places in the area of Environmental and Human Health Risk Assessment of Persistent Toxic Substance. Y. Zhang acknowledges the financial support from the National Natural Science Foundation of China (21045004). We would express our sincere thanks to Ms Silva T. Mo of the Department of Chemistry, Hong Kong Baptist University for acquiring the MALDI-TOF MS. Part of this work was presented at The 18th Symposium on Chemistry Postgraduate Research in Hong Kong on 30th April 2011.",

year = "2012",

month = sep,

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doi = "10.1039/c2nr30984j",

language = "English",

volume = "4",

pages = "5325--5332",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - High-performance liquid chromatographic analysis of as-synthesised N,N′-dimethylformamide-stabilised gold nanoclusters product

AU - Xie, Shunping

AU - Paau, Man Chin

AU - Zhang, Yan

AU - Shuang, Shaomin

AU - Chan, Wan

AU - Choi, Martin M.F.

N1 - Financial supports from the Hundred Talent Programme of Shanxi Province and HKBU Faculty Research Grant (FRG2/10-11/112) are gratefully acknowledged. M. C. Paau acknowledges the receipt of a postgraduate studentship from the Additional UGC-funded Research Postgraduate Places in the area of Environmental and Human Health Risk Assessment of Persistent Toxic Substance. Y. Zhang acknowledges the financial support from the National Natural Science Foundation of China (21045004). We would express our sincere thanks to Ms Silva T. Mo of the Department of Chemistry, Hong Kong Baptist University for acquiring the MALDI-TOF MS. Part of this work was presented at The 18th Symposium on Chemistry Postgraduate Research in Hong Kong on 30th April 2011.

PY - 2012/9/7

Y1 - 2012/9/7

N2 - Reverse-phase high-performance liquid chromatographic (RP-HPLC) separation and analysis of polydisperse water-soluble gold nanoclusters (AuNCs) stabilised with N,N′-dimethylformamide (DMF) were investigated. Under optimal elution gradient conditions, the separation of DMF-AuNCs was monitored by absorption and fluorescence spectroscopy. The UV-vis spectral characteristics of the separated DMF-AuNCs have been captured and they do not possess distinct surface plasmon resonance bands, indicating that all DMF-AuNCs are small AuNCs. The photoluminescence emission spectra of the separated DMF-AuNCs are in the blue-light region. Moreover, cationic DMF-AuNCs are for the first time identified by ion chromatography. Our proposed RP-HPLC methodology has been successfully applied to separate AuNCs of various Au atoms as well as DMF-stabilised ligands. Finally, the composition of the separated DMF-AuNCs was confirmed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and electrospray ionisation mass spectrometry, proving that the as-synthesised DMF-AuNCs product consists of Au10+, Au10, Au11, Au12, Au13, and Au 14 NCs stabilised with various numbers of DMF ligands.

AB - Reverse-phase high-performance liquid chromatographic (RP-HPLC) separation and analysis of polydisperse water-soluble gold nanoclusters (AuNCs) stabilised with N,N′-dimethylformamide (DMF) were investigated. Under optimal elution gradient conditions, the separation of DMF-AuNCs was monitored by absorption and fluorescence spectroscopy. The UV-vis spectral characteristics of the separated DMF-AuNCs have been captured and they do not possess distinct surface plasmon resonance bands, indicating that all DMF-AuNCs are small AuNCs. The photoluminescence emission spectra of the separated DMF-AuNCs are in the blue-light region. Moreover, cationic DMF-AuNCs are for the first time identified by ion chromatography. Our proposed RP-HPLC methodology has been successfully applied to separate AuNCs of various Au atoms as well as DMF-stabilised ligands. Finally, the composition of the separated DMF-AuNCs was confirmed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and electrospray ionisation mass spectrometry, proving that the as-synthesised DMF-AuNCs product consists of Au10+, Au10, Au11, Au12, Au13, and Au 14 NCs stabilised with various numbers of DMF ligands.

UR - https://www.scopus.com/pages/publications/84865176497

U2 - 10.1039/c2nr30984j

DO - 10.1039/c2nr30984j

M3 - Journal article

AN - SCOPUS:84865176497

SN - 2040-3364

VL - 4

SP - 5325

EP - 5332

JO - Nanoscale

JF - Nanoscale

IS - 17

ER -

High-performance liquid chromatographic analysis of as-synthesised N,N′-dimethylformamide-stabilised gold nanoclusters product

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